New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe

PLoS Genetics
Takahisa MakiGenevieve Thon

Abstract

Mating-type switching in Schizosaccharomyces pombe entails programmed gene conversion events regulated by DNA replication, heterochromatin, and the HP1-like chromodomain protein Swi6. The whole mechanism remains to be fully understood. Using a gene deletion library, we screened ~ 3400 mutants for defects in the donor selection step where a heterochromatic locus, mat2-P or mat3-M, is chosen to convert the expressed mat1 locus. By measuring the biases in mat1 content that result from faulty directionality, we identified in total 20 factors required for donor selection. Unexpectedly, these included the histone H3 lysine 4 (H3K4) methyltransferase complex subunits Set1, Swd1, Swd2, Swd3, Spf1 and Ash2, the BRE1-like ubiquitin ligase Brl2 and the Elongator complex subunit Elp6. The mutant defects were investigated in strains with reversed donor loci (mat2-M mat3-P) or when the SRE2 and SRE3 recombination enhancers, adjacent to the donors, were deleted or transposed. Mutants in Set1C, Brl2 or Elp6 altered balanced donor usage away from mat2 and the SRE2 enhancer, towards mat3 and the SRE3 enhancer. The defects in these mutants were qualitatively similar to heterochromatin mutants lacking Swi6, the NAD+-dependent histone deacetylase S...Continue Reading

References

Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R EgelA J Klar
Jan 10, 2002·Nature Cell Biology·Nobuhiro NonakaYoshinori Watanabe
Jan 26, 2002·Molecular Biology of the Cell·Satoshi KatayamaTakashi Toda
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ken-ichi Noma, Shiv I S Grewal
Sep 7, 2002·Science·Ira M HallShiv I S Grewal
Dec 19, 2002·The Journal of Biological Chemistry·Assen RoguevA Francis Stewart
Jul 18, 2003·Current Biology : CB·Gurumurthy D ShankaranarayanaShiv I S Grewal
Dec 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yufuko AkamatsuHiroshi Iwasaki
Apr 3, 2004·Genes & Development·Sonya Vengrova, Jacob Z Dalgaard
Apr 9, 2004·The EMBO Journal·Julie SollierChristophe de La Roche Saint-André
Nov 17, 2004·Genetics·Lisa L Freeman-CookPatricia Laurenson
Dec 17, 2004·Molecular and Cellular Biology·Allyson M HolmesBenoit Arcangioli
Nov 22, 2005·EMBO Reports·Sonya Vengrova, Jacob Z Dalgaard
Feb 16, 2006·Molecular and Cellular Biology·Stephanie PebernardMichael N Boddy
Aug 22, 2006·Nature Structural & Molecular Biology·Nami HarutaHiroshi Iwasaki
Sep 26, 2006·Biochemical and Biophysical Research Communications·Yasmine M MamnunTakashi Toda
Feb 10, 2007·Cell·Tomoyasu SugiyamaShiv I S Grewal
Mar 17, 2007·The Journal of Biological Chemistry·Martin Zofall, Shiv I S Grewal
Sep 1, 2007·The EMBO Journal·John PruddenMichael N Boddy

❮ Previous
Next ❯

Citations

Nov 2, 2018·Current Genetics·Geneviève ThonHiroshi Iwasaki
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Sebastian Jespersen CharltonGeneviève Thon
Dec 14, 2021·ELife·José Fabricio López HernándezSarah E Zanders

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
fluorescence microscopy
histone acetylation
acetylation
gene knockout
fluorescence

Software Mentioned

ImageQuant
Acapella

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.