PMID: 2508486Oct 1, 1989Paper

New insights into lactase and glycosylceramidase activities of rat lactase-phlorizin hydrolase

The American Journal of Physiology
H A BüllerR J Grand

Abstract

Lactase-phlorizin hydrolase, a small intestinal disaccharidase, has been considered mainly an enzyme important only for the hydrolysis of lactose. After weaning in most mammals lactase-specific activity falls markedly, and, functionally, adult mammals are considered to be lactase deficient. However, the persistence of low levels of lactase activity in adulthood has never been explained. In addition, it has been suggested that lactase-phlorizin hydrolase is associated with glycosylceramidase activity when the enzyme is prepared by column chromatography, but it is unclear whether this represents copurified activities or two catalytic sites on one peptide. The developmental patterns of lactase-phlorizin hydrolase and other disaccharidases were investigated in homogenates of total rat small intestine; lactase and several glycosylceramidases were measured in immunoprecipitates from these homogenates using a monoclonal antibody. The developmental pattern of total lactase activity showed a steady 2.3-fold increase to adult levels (specific activity decreased eightfold), whereas total phlorizin-hydrolase activity increased 10.7-fold (specific activity decreased threefold). As expected, levels of both total and specific sucrase and malt...Continue Reading

Citations

Apr 4, 2017·Journal of Nutritional Science and Vitaminology·Aoi FujiiTatsuya Sugawara
Jul 26, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·S LukovacH J Verkade
Jan 29, 2021·Journal of Agricultural and Food Chemistry·Kazushi OhtaTatsuya Sugawara

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