New insights into mechanisms of material ejection in MALDI mass spectrometry for a wide range of spot sizes

Scientific Reports
Marcel Niehaus, Jens Soltwisch

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for the analysis of large biomolecules in numerous applications. The technique utilizes nanosecond-long laser pulses at various spot sizes to eject and ionize large molecules embedded in a highly absorptive chemical matrix. Despite the methods name, 'molecular desorption' from the matrix crystal surface is not the sole mechanism discussed for material ejection in MALDI, but additional ablation of larger clusters has been reported. Here we present results on the influence of laser fluence and spot size on the mechanisms of the initial material ejection in MALDI and subsequent plume development. We used a laser-based postionization (MALDI-2) as well as a complementary photoacoustic method to monitor the material ejection step. The photoacoustic data reveal a quasi-thermal sublimation process up to a transition fluence. Above this threshold fluence additional ablation processes are observed. Complementary investigations on plume dynamics by MALDI-2 showed an ejection of predominantly fast particles for desorption conditions while ablation produces considerably slower ejecta. Additionally the presented results revealed a peculiar influence of th...Continue Reading

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Citations

Apr 6, 2019·Journal of the American Society for Mass Spectrometry·Kenneth N RobinsonJosephine Bunch
Feb 21, 2019·Annual Review of Analytical Chemistry·Luke HanleyYeni P Yung
Jan 1, 2019·Mass Spectrometry·Britt S R ClaesRon M A Heeren
Nov 12, 2020·Mass Spectrometry Reviews·Wendy H MüllerGauthier Eppe
Mar 4, 2021·Journal of the American Society for Mass Spectrometry·Michael J TaylorChristopher R Anderton
Mar 30, 2021·Journal of the American Society for Mass Spectrometry·Eike U BrockmannKlaus Dreisewerd
May 29, 2021·Journal of the American Society for Mass Spectrometry·Shabarinath NambiarJoel P A Gummer
Sep 26, 2020·Analytical Chemistry·Bram HeijsKlaus Dreisewerd
Apr 3, 2020·Journal of the American Society for Mass Spectrometry·Hou-Yu LinChi-Kung Ni
Mar 10, 2020·Analytical Chemistry·Marcel S Boskamp, Jens Soltwisch
Aug 25, 2020·The Journal of Physical Chemistry. a·Michael J ApsokarduMurray V Johnston
Jul 17, 2020·Journal of the American Society for Mass Spectrometry·Alexander PotthoffJens Soltwisch
Sep 19, 2020·Journal of the American Society for Mass Spectrometry·Marcel NiehausJosephine Bunch

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versaScan
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