New insights into melatonin/CRH signaling in hamster Leydig cells

General and Comparative Endocrinology
Soledad Paola RossiMónica B Frungieri

Abstract

We have previously described that melatonin inhibits androgen production in hamster testes via melatonin subtype 1a (mel1a) receptors and the local corticotrophin-releasing hormone (CRH) system. This study attempted to determine the initial events of the melatonin/CRH signaling pathway. In Leydig cells from reproductively active Syrian hamsters, Western blotting, reverse transcription quantitative polymerase chain reaction (RT-qPCR) and a colorimetric assay demonstrated that melatonin and CRH activate tyrosine phosphatases and subsequently reduce the phosphorylation levels of extracellular signal-regulated kinase (erk) and c-jun N-terminal kinase (jnk), down-regulate the expression of c-jun, c-fos and steroidogenic acute regulatory (StAR), and inhibit the production of testosterone. These effects were prevented by a highly selective CRH antagonist, thus indicating that melatonin does not exert a direct role. Specific mitogen-activated protein kinase kinase (MEK) and jnk blockers inhibited expression of c-jun, c-fos, StAR and the production of testosterone, confirming that these are events triggered downstream of erk and jnk. In Leydig cells from photoperiodically regressed adult hamsters, CRH inhibited the production of androst...Continue Reading

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Citations

Sep 11, 2014·Molecular Human Reproduction·Cátia S RochaMarco G Alves
Apr 29, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chunjin Li, Xu Zhou
Jan 9, 2016·Andrology·M Gonzalez-ArtoA Casao
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Nov 2, 2021·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Farideh Zafari ZangenehMasoumeh Masoumi

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