New Insights into the Anti-Inflammatory and Antioxidant Properties of Nitrated Phospholipids

Lipids
Tânia MeloMaria Rosario M Domingues

Abstract

Nitro-fatty acids (NO2 -FA) have been widely studied with regard to their identification, structural characterization, and biological actions. NO2 -FA could also be present endogenously esterified to phospholipids (PL), and NO2 -PL were already detected in cardiac mitochondria from diabetic rats and cardiomyoblasts subjected to starvation. However, the biological actions of NO2 -PL have been overlooked. In this study, we evaluate the antioxidant and anti-inflammatory potential of the nitrated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) formed in vitro by incubation with NO2 BF4 , in a well-recognized mimetic model of nitroxidative stress. Nitrated POPC showed anti-radical ability to reduce both 2,2-diphenyl-1-picrylhydrazyl radical (DPPH• ) (IC20 = 225 ± 4 μg/mL; Trolox equivalent (TE) = 86 ± 6 μmol Trolox/g lipid) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS•+ ) (IC50 = 124 ± 2 μg/mL; TE = 152 ± 9 μmol Trolox/g lipid). Also, higher lag times were achieved in oxygen radical absorbance capacity (ORAC) assay for nitrated POPC, indicating a faster reaction with oxygen-derived radicals (TE = 1.03 ± 0.22 and TE = 1.30 ± 0.16 mmol Trolox/g lipid for nonmodified and nitrated POPC, respecti...Continue Reading

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Citations

Jul 25, 2019·Journal of Experimental Botany·Magdalena Arasimowicz-Jelonek, Jolanta Floryszak-Wieczorek
Aug 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Diana LopesMaria Rosário Domingues
Jan 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bruna NevesTânia Melo
May 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bruna NevesTânia Melo
Aug 24, 2020·Archives of Biochemistry and Biophysics·Maria C OliveiraRodrigo M Cordeiro
Jun 15, 2019·Free Radical Biology & Medicine·Sofia DuarteDolores Pérez-Sala
Feb 10, 2021·Current Molecular Pharmacology·Varsha KomallaMehra Haghi

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