New Insights into the Classification and Integration Specificity of Streptococcus Integrative Conjugative Elements through Extensive Genome Exploration

Frontiers in Microbiology
Chloé AmbrosetNathalie Leblond-Bourget

Abstract

Recent genome analyses suggest that integrative and conjugative elements (ICEs) are widespread in bacterial genomes and therefore play an essential role in horizontal transfer. However, only a few of these elements are precisely characterized and correctly delineated within sequenced bacterial genomes. Even though previous analysis showed the presence of ICEs in some species of Streptococci, the global prevalence and diversity of ICEs was not analyzed in this genus. In this study, we searched for ICEs in the completely sequenced genomes of 124 strains belonging to 27 streptococcal species. These exhaustive analyses revealed 105 putative ICEs and 26 slightly decayed elements whose limits were assessed and whose insertion site was identified. These ICEs were grouped in seven distinct unrelated or distantly related families, according to their conjugation modules. Integration of these streptococcal ICEs is catalyzed either by a site-specific tyrosine integrase, a low-specificity tyrosine integrase, a site-specific single serine integrase, a triplet of site-specific serine integrases or a DDE transposase. Analysis of their integration site led to the detection of 18 target-genes for streptococcal ICE insertion including eight that ...Continue Reading

References

Jan 1, 1986·Annual Review of Microbiology·D B Clewell, C Gawron-Burke
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·N E Murray
Oct 6, 2000·Annual Review of Microbiology·J Hacker, J B Kaper
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Jan 19, 2002·Plasmid·Ariane Toussaint, Christophe Merlin
Jun 5, 2002·Trends in Genetics : TIG·Eduardo P C Rocha, Antoine Danchin
Nov 2, 2002·Molecular Microbiology·Vincent BurrusGérard Guédon
Jul 10, 2003·Current Opinion in Biotechnology·Jan Roelof van der Meer, Vladimir Sentchilo
Dec 23, 2003·Journal of Molecular Biology·Amy C Groth, Michele P Calos
Mar 24, 2004·Nature Reviews. Microbiology·Timothy J Mitchell
Apr 22, 2004·Nature Reviews. Microbiology·Ulrich DobrindtJörg Hacker
Apr 3, 2007·International Journal of Medical Microbiology : IJMM·Werner Köhler
Nov 8, 2008·Molecular Microbiology·Zoe HeatherAndrew S Waller
Jan 31, 2009·FEMS Microbiology Reviews·Mario JuhasDerrick W Crook
Feb 3, 2009·Molecular Microbiology·Mathieu BrochetPhilippe Glaser
Oct 14, 2009·BMC Bioinformatics·Van Hoa Nguyen, Dominique Lavenier
Dec 1, 2009·Molecular Microbiology·Catherine A LeeAlan D Grossman
Jan 14, 2010·Biochemical and Biophysical Research Communications·Clive PersaudMichael O'Connor
Jul 6, 2010·Nature Reviews. Microbiology·Rachel A F Wozniak, Matthew K Waldor
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Chris SmillieFernando de la Cruz
Jan 6, 2011·FEMS Microbiology Letters·Adrian L CooksonGraeme T Attwood
Jan 26, 2011·Antimicrobial Agents and Chemotherapy·Marina MingoiaMaria Pia Montanari
Jun 11, 2011·FEMS Microbiology Reviews·Adam P Roberts, Peter Mullany
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins

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Citations

Apr 23, 2017·Applied and Environmental Microbiology·Narimane DahmaneSophie Payot
Sep 9, 2020·Applied Microbiology and Biotechnology·Li YiYang Wang
Aug 16, 2018·Frontiers in Microbiology·Francesco SantoroFrancesco Iannelli
Sep 29, 2020·Frontiers in Microbiology·Chiara CrestaniRuth N Zadoks
Apr 13, 2021·Frontiers in Microbiology·Mingyu YangYanlin Zhao
May 22, 2019·Microbiology Spectrum·Francesco SantoroGianni Pozzi
Mar 31, 2018·Microbiology Spectrum·Marisa HaenniJean-Yves Madec
Sep 1, 2021·Scientific Reports·Davide PorcellatoOddvar Oppegaard

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Methods Mentioned

BETA
ICEs
ICE
GTPase

Software Mentioned

CD
BlastClust
ngKlast
Biomart
BlastP
Artemis
ICEberg
Insyght
T4SSscan
multifasta

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