Abstract
To evaluate the effects of SR-16234 (SR), a selective estrogen receptor modulator (SERM), on murine endometriosis-like lesions. BALB/c mice (n = 53) were used to establish the murine endometriosis model. Ovariectomized, estradiol replaced, 6-week-old murine endometriosis model were injected with lipopolysaccharide (LPS) with or without SR (1 mg/kg/d) or vehicle, over a period of 4 weeks. Upon treatment completion, the endometriosis-like lesions that developed in the abdominal cavity of mice were counted, measured, and collected. Gene expression of inflammatory cytokines and estrogen receptor (ER) in the lesions was assessed by real-time RT-PCR. Immunohistochemical analysis was used to evaluate the effect of SR on cell proliferation, angiogenic activity, inflammation, and NF-κB phosphorylation. Treatment with SR significantly reduced the total number and size of lesions per mouse without inducing endometrial growth. In addition, SR downregulated LPS-enhanced Vegf, Il-6, Ptgs-2, and Ccl-2 and ER mRNA expression in endometriosis-like lesions. Immunohistochemical analysis demonstrated a decrease in percentage of positive cells of Ki67, and intensity and rate of positive cells of ERα, CD3, F4/80, PECAM by SR treatment. SR also decre...Continue Reading
References
Jul 5, 2001·Fertility and Sterility·T HaradaN Terakawa
Feb 8, 2003·The Journal of Clinical Endocrinology and Metabolism·Yasuko SakamotoNaoki Terakawa
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·J KitawakiH Honjo
Dec 19, 2003·Biochemical and Biophysical Research Communications·Yasuji YamamotoShigeaki Kato
Jul 9, 2005·Endocrinology·Andrew G GeiserJeffrey A Dodge
Aug 30, 2005·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Z YaoK Vakerich
Nov 21, 2007·Gynecologic and Obstetric Investigation·Reinaldo González-RamosJacques Donnez
Dec 1, 2007·Endocrinology·Orhan BukulmezCarole R Mendelson
Jan 1, 2008·Obstetrics and Gynecology·Pamela StrattonLynnette K Nieman
Jan 16, 2009·The New England Journal of Medicine·Serdar E Bulun
Jan 20, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·T SaekiT Ikeda
Jan 30, 2009·Basic & Clinical Pharmacology & Toxicology·Yuji KumagaiHideo Tsuruta
Aug 4, 2009·Fertility and Sterility·Yasuko TakenakaTasuku Harada
Sep 1, 2007·Women's Health·Peter SimsaThomas M D'Hooghe
Mar 2, 2010·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Devrim AltintasMehmet B Cetinkaya
Jul 16, 2010·Fertility and Sterility·Khaleque Newaz KhanHideaki Masuzaki
Jul 22, 2011·Cancer Treatment Reviews·Javaid IqbalSteven A Narod
Jun 9, 2012·Cancer Science·Hideo InajiShinzaburo Noguchi
Jun 16, 2012·Endocrinology·Katherine A BurnsKenneth S Korach
Jul 24, 2012·Fertility and Sterility·Richard O Burney, Linda C Giudice
Aug 8, 2012·Therapeutic Advances in Musculoskeletal Disease·Barry S Komm, Arkadi A Chines
Oct 16, 2012·Current Clinical Pharmacology·Philipp Y MaximovV Craig Jordan
Dec 21, 2012·Endocrinology·Yan SongWei Yue
Dec 29, 2012·Menopause : the Journal of the North American Menopause Society·Jennifer A HarveySebastian Mirkin
Jul 24, 2013·Fertility and Sterility·Eri TakaiTasuku Harada
Aug 15, 2013·Obstetrical & Gynecological Survey·Salvatore GizzoGiovanni Battista Nardelli
Nov 20, 2013·Maturitas·Sebastian MirkinBarry S Komm
Apr 18, 2014·Biology of Reproduction·Hanyia NaqviHugh S Taylor
Jun 10, 2014·The American Journal of Pathology·Erin GreavesPhilippa T K Saunders
Oct 31, 2014·Reproductive Sciences·Khaleque Newaz KhanHideaki Masuzaki
Jan 23, 2015·Science Translational Medicine·Yuechao ZhaoBenita S Katzenellenbogen
Nov 7, 2015·Cell·Sang Jun HanBert W O'Malley
Dec 26, 2015·Reproductive Sciences·Masao IzawaTasuku Harada
Feb 1, 2017·American Journal of Reproductive Immunology : AJRI·Yukihiro AzumaTasuku Harada
Feb 1, 2017·Fertility and Sterility·Mohamed A BedaiwyRobert Casper
Jun 24, 2017·Nature Communications·Sandra AnderssonAnna Asplund