New Insights into the Growth and Transformation of Vesicles: A Free-Flow Electrophoresis Study

The Journal of Physical Chemistry. B
Tereza Pereira de SouzaPier Luigi Luisi

Abstract

The spontaneous formation of lipid vesicles, in particular fatty acid vesicles, is considered an important physical process at the roots of cellular life. It has been demonstrated previously that the addition of fatty acid micelles to preformed vesicles induces vesicle self-reproduction by a growth-division mechanism. Despite multiple experimental efforts, it remains unresolved how vesicles rearrange upon the addition of fresh membrane-forming compounds, and whether solutes that are initially encapsulated inside the mother vesicles are evenly redistributed among the daughter ones. Here we investigate the growth-division of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) vesicles, which, following the addition of oleate micelles, form mixed oleate/POPC vesicles. Our approach is based on free-flow electrophoresis (FFE) and cryogenic transmission electronmicroscopy (cryo-TEM). Two new features emerge from this study. FFE analysis unexpectedly reveals that the uptake of oleate micelles by POPC vesicles follows two different pathways depending on the micelles/vesicles ratio. At low oleate molar fractions (<0.35), plain incorporation of oleate into pre-existing POPC vesicles is our dominant observation. In contrast, olea...Continue Reading

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Citations

Sep 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jaime Ortega-ArroyoStephen P Fletcher
Jul 11, 2020·Advanced Biosystems·Ivan IvanovRumiana Dimova
Jan 26, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Martín E VillanuevaRaquel V Vico

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