New insights into the inflamed tumor immune microenvironment of gastric cancer with lymphoid stroma: from morphology and digital analysis to gene expression

Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
Irene GulloFátima Carneiro

Abstract

Gastric cancer with lymphoid stroma (GCLS) is characterized by prominent stromal infiltration of T-lymphocytes. The aim of this study was to investigate GCLS biology through analysis of clinicopathological features, EBV infection, microsatellite instability (MSI), immune gene-expression profiling and PD-L1 status in neoplastic cells and tumor immune microenvironment. Twenty-four GCLSs were analyzed by RNA in situ hybridization for EBV (EBER), PCR/fragment analysis for MSI, immunohistochemistry (PD-L1, cytokeratin, CD3, CD8), co-immunofluorescence (CK/PD-L1, CD68/PD-L1), NanoString gene-expression assay for immune-related genes and PD-L1 copy number alterations. CD3+ and CD8+ T-cell densities were calculated by digital analysis. Fifty-four non-GCLSs were used as control group. GCLSs displayed distinctive clinicopathological features, such as lower pTNM stage (p = 0.02) and better overall survival (p = 0.01). EBV+ or MSI-high phenotype was found in 66.7 and 16.7% cases, respectively. GCLSs harbored a cytotoxic T-cell-inflamed profile, particularly at the invasive front of tumors (p < 0.01) and in EBV+ cases (p = 0.01). EBV+ GCLSs, when compared to EBV- GCLSs, showed higher mRNA expression of genes related to Th1/cytotoxic and imm...Continue Reading

References

Sep 30, 2000·Histopathology·M S ChangY I Kim
Jul 16, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Karen L GroggThomas C Smyrk
Aug 13, 2009·Virchows Archiv : an International Journal of Pathology·Enrico SolciaOmbretta Luinetti
Feb 25, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christy RalphFiona C Thistlethwaite
Sep 29, 2011·Pathology, Research and Practice·Masashi Fukayama, Tetsuo Ushiku
Mar 16, 2012·Nature Reviews. Cancer·Wolf Herman FridmanJérôme Galon
Jun 5, 2012·The New England Journal of Medicine·Suzanne L TopalianMario Sznol
Aug 30, 2013·Science Translational Medicine·Stefani SprangerThomas F Gajewski
Feb 4, 2014·Virchows Archiv : an International Journal of Pathology·Cecília DurãesFátima Carneiro
Apr 29, 2014·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Na ChengChun-Kui Shao
Aug 1, 2014·Nature·UNKNOWN Cancer Genome Atlas Research Network
Jan 17, 2015·The Journal of Surgical Research·Sungmin ParkSung Kim
Jan 31, 2015·International Journal of Oncology·Aya Shinozaki-UshikuMasashi Fukayama
Apr 29, 2015·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Hyun LimByung Sik Kim
Mar 25, 2016·Oncotarget·Christine BögerChristoph Röcken
Apr 2, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Namrata SetiaGregory Y Lauwers
Apr 4, 2016·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Markus MoehlerMichel Ducreux
Jun 10, 2016·Nature·Keisuke KataokaSeishi Ogawa
Sep 16, 2016·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Akihito KawazoeAtsushi Ochiai
Nov 3, 2016·The New England Journal of Medicine·Vassiliki A Boussiotis
Dec 10, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Ruri SaitoMasashi Fukayama
Jan 6, 2017·Current Medical Research and Opinion·Burak BilginBülent Yalçın
Jan 20, 2017·Nature·Daniel S Chen, Ira Mellman
May 2, 2017·The Journal of Surgical Research·Marcus Fernando Kodama Pertille RamosUlysses Ribeiro Junior
Jul 28, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bo Hwa SohnJu-Seog Lee
Oct 25, 2017·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Alexander BaraniskinWolff Schmiegel

❮ Previous
Next ❯

Citations

Jul 19, 2018·International Journal of Molecular Sciences·Irene GulloPatrícia Oliveira
Dec 15, 2019·Virchows Archiv : an International Journal of Pathology·Masashi FukayamaAtsushi Kaneda
Jan 30, 2020·The Journal of Pathology·Ralf Huss, Sarah E Coupland
Jan 1, 2021·Histopathology·Irene GulloFátima Carneiro
Oct 27, 2020·Frontiers in Oncology·Evgeny N ImyanitovIlya V Tsimafeyeu
Apr 24, 2020·Journal of the National Cancer Institute·Benjamin R ChallonerMarco Gerlinger
Sep 13, 2020·Helicobacter·Carlos ResendeJose Carlos Machado
Mar 7, 2021·International Journal of Molecular Sciences·Dita UlaseChristoph Röcken
Jun 3, 2021·Cancers·Yuichiro HatanoAkira Hara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.