New insights into the interaction of centrin with Sfi1

Biochimica Et Biophysica Acta
Juan Martinez-Sanz, Liliane Assairi

Abstract

Centrin binds to Rad4(XPC) and Sfi1 through the hydrophobic motif W(1)xxL(4)xxxL(8) in the opposite orientation. Rad4 has one motif, but Sfi1 has approximately 20 repeats, each of which interacts with a centrin molecule. To investigate the parameters involved in centrin binding, we purified a ScSfi1 domain containing 6 repeats complexed with either yeast centrin Cdc31 or human centrin 1. The present study was performed using mutagenesis of centrin and of Sfi1 residues involved in centrin binding and the stability of the centrin-centrin complexes was assessed using thermal denaturation and CD. Calcium stabilized these complexes, as indicated by the Tm increases measured by circular dichroism. The complexes, which were composed of Sfi1 variants and yeast centrin, were analysed in the presence of EDTA. The replacement of W with F within the repeat region yielded a functional repeat (Tm 45°C). The replacement of W with A in two adjacent Sfi1 repeats reduced the thermal stability of the Sfi1-centrin complexes (40°C). We analysed three HsCen1 variants that were homologous to the yeast mutants and induced cell cycle arrest during the G2/M transition. The HsCen1 variants E105K and F113L reduced the thermal stability (50°C, 50°C) of the...Continue Reading

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Citations

Jul 19, 2017·The Journal of Eukaryotic Microbiology·Helmut Plattner
Jan 29, 2019·Frontiers in Genetics·Roberta Fraschini

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