New insights into the proteomic characterization of the coral snake Micrurus pyrrhocryptus venom

Toxicon : Official Journal of the International Society on Toxinology
Timoteo Olamendi-PortugalL D Possani

Abstract

A proteomic analysis of the soluble venom of the coral snake Micrurus pyrrhocryptus is reported in this work. The whole soluble venom was separated by RP-HPLC and the molecular weights of its components (over 100) were determined by mass spectrometry. Three main sets of components were identified, corresponding to peptides with molecular masses from 5 to 8 kDa, proteins from 12 to 16 kDa and proteins from 20 to 30 kDa. Two components were fully sequenced: one α-neurotoxic peptide of 7210 Da with slight blocking activity of the nicotinic acetylcholine receptor (nAChR) and a phospholipase A2 (PLA2) with molecular weight 13517 Da and no effect on the nAChR. PLA2 activity was evaluated for all RP-HPLC components. In addition, N-terminal sequence was obtained for eleven components using Edman degradation. Among these, three were similar to known PLA2's, six to three-finger toxins (3FTx) and one to Kunitz-type serine protease inhibitors. Two-dimensional gel electrophoresis of the venom allowed the separation of about thirty spots with components of molecular weights from 25 to 70 kDa. Seventeen spots were recovered from the gel, digested with trypsin and the corresponding peptides (85) were sequenced by MS/MS allowing identification ...Continue Reading

Citations

Nov 18, 2018·Toxins·Aisha MunawarChristian Betzel
Feb 10, 2021·Cardiovascular Toxicology·Lilian de Paula Gonçalves ReisMarília Martins Melo
Nov 17, 2020·Toxicology Letters·Daniel DashevskyBryan G Fry
Aug 7, 2020·Toxicon : Official Journal of the International Society on Toxinology·Rafael S FlorianoEdward G Rowan
Jul 26, 2021·Toxicon : Official Journal of the International Society on Toxinology·Adolfo Rafael de RoodtAlejandro Alagón

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