New Insights into the Regulation of Cell-Surface Signaling Activity Acquired from a Mutagenesis Screen of the Pseudomonas putida IutY Sigma/Anti-Sigma Factor

Frontiers in Microbiology
Karlijn C BastiaansenMaría A Llamas

Abstract

Cell-surface signaling (CSS) is a signal transfer system that allows Gram-negative bacteria to detect environmental signals and generate a cytosolic response. These systems are composed of an outer membrane receptor that senses the inducing signal, an extracytoplasmic function sigma factor (σ(ECF)) that targets the cytosolic response by modifying gene expression and a cytoplasmic membrane anti-sigma factor that keeps the σ(ECF) in an inactive state in the absence of the signal and transduces its presence from the outer membrane to the cytosol. Although CSS systems regulate bacterial processes as crucial as stress response, iron scavenging and virulence, the exact mechanisms that drive CSS are still not completely understood. Binding of the signal to the CSS receptor is known to trigger a signaling cascade that results in the regulated proteolysis of the anti-sigma factor and the activation of the σ(ECF) in the cytosol. This study was carried out to generate new insights in the proteolytic activation of CSS σ(ECF). We performed a random mutagenesis screen of the unique IutY protein of Pseudomonas putida, a protein that combines a cytosolic σ(ECF) domain and a periplasmic anti-sigma factor domain in a single polypeptide. In respo...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K R SilberR T Sauer
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F C FranklinK N Timmis
Aug 1, 1995·Protein Science : a Publication of the Protein Society·K C KeilerR T Sauer
Feb 2, 1996·The Journal of Biological Chemistry·K C Keiler, R T Sauer
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Iain L LamontMichael L Vasil
Jan 22, 2003·Environmental Microbiology·Pierre Cornelis, Sandra Matthijs
Jan 24, 2003·Genome Biology·Mark S B Paget, John D Helmann
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Keith Poole, Geoffery A McKay
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Oct 19, 2004·Annual Review of Microbiology·Cécile Wandersman, Philippe Delepelaire
Dec 17, 2004·Nucleic Acids Research·Benjamin D Biles, Bernard A Connolly
Apr 26, 2005·Nucleic Acids Research·Yang Zhang, Jeffrey Skolnick
Jul 5, 2005·Trends in Microbiology·Ralf Koebnik
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Volkmar BraunAnnette Sauter
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Brent O Cezairliyan, Robert T Sauer
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dongru QiuHongwei D Yu
Dec 19, 2007·Molecular Microbiology·María A LlamasWilbert Bitter
Nov 6, 2008·Current Opinion in Microbiology·Sarah E Ades
Feb 28, 2009·Nature Protocols·Lawrence A Kelley, Michael J E Sternberg
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xiaochun LiYigong Shi
Nov 6, 2009·Molecular Microbiology·Karla A Mettrick, Iain L Lamont
Apr 28, 2010·Annual Review of Microbiology·Nicholas NoinajSusan K Buchanan
May 5, 2010·Nucleic Acids Research·Mickael GoujonRodrigo Lopez
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström
Jun 1, 2010·Protein Science : a Publication of the Protein Society·Dong Young KimKyeong Kyu Kim
Aug 1, 2009·Environmental Microbiology Reports·Pierre Cornelis, Josselin Bodilis

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Citations

Jun 18, 2019·Molecular Microbiology·Joaquín R Otero-AsmanMaría A Llamas
Aug 8, 2019·Environmental Microbiology·Joaquín R Otero-AsmanMaría A Llamas
Nov 17, 2020·Frontiers in Molecular Biosciences·Sarah Wettstadt, María A Llamas

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Methods Mentioned

BETA
PCR
RIP
pull-down
interaction studies

Software Mentioned

Phyre2
align
DALI
Protein Homology / analogy Recognition Engine ( Phyre2 )
ClustalW
TM

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