New insights into the spring-loaded conformational change of influenza virus hemagglutinin

Journal of Virology
Jennifer A GruenkeJudith M White

Abstract

Influenza virus hemagglutinin undergoes a conformational change in which a loop-to-helix "spring-loaded" conformational change forms a coiled coil that positions the fusion peptide for interaction with the target bilayer. Previous work has shown that two proline mutations designed to disrupt this change disrupt fusion but did not determine the basis for the fusion defect. In this work, we made six additional mutants with single proline substitutions in the region that undergoes the spring-loaded conformational change and two additional mutants with double proline substitutions in this region. All double mutants were fusion inactive. We analyzed one double mutant, F63P/F70P, as an example. We observed that F63P/F70P undergoes key low-pH-induced conformational changes and binds tightly to target membranes. However, limited proteolysis and electron microscopy observations showed that the mutant forms a coiled coil that is only approximately 50% the length of the wild type, suggesting that it is splayed in its N-terminal half. This work further supports the hypothesis that the spring-loaded conformational change is necessary for fusion. Our data also indicate that the spring-loaded conformational change has another role beyond pres...Continue Reading

References

Dec 10, 1990·FEBS Letters·J BentzD Alford
Dec 17, 1990·FEBS Letters·D N Woolfson, D H Williams
Sep 1, 1995·Trends in Biochemical Sciences·R A Sayle, E J Milner-White
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·J M White
Jan 1, 1996·Annual Review of Cell and Developmental Biology·L D HernandezJ M White
May 22, 1997·Nature·W WeissenhornD C Wiley
Feb 4, 1998·Biophysical Journal·T ShangguanJ Bentz
Feb 12, 1999·Journal of Molecular Biology·R F EpandR M Epand
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·J ChenD C Wiley
Feb 2, 2000·Biophysical Journal·J Bentz

❮ Previous
Next ❯

Citations

Nov 21, 2007·Pflügers Archiv : European journal of physiology·Ayana HintonMichael Forgac
Oct 28, 2011·Cell Death & Disease·D Gonzalez-HalphenM D Esposti
Mar 27, 2004·Nature Reviews. Microbiology·Dimiter S Dimitrov
Oct 7, 2003·Annual Review of Biochemistry·Leonid V Chernomordik, Michael M Kozlov
May 23, 2015·The Journal of Physical Chemistry. B·Javier L Baylon, Emad Tajkhorshid
Mar 5, 2016·Biochimica Et Biophysica Acta·Javier L BaylonEmad Tajkhorshid
Jul 23, 2003·Biochimica Et Biophysica Acta·Joe Bentz, Aditya Mittal
Oct 16, 2009·The Journal of General Virology·Marie-Cécile GiocondiChristian Le Grimellec
Mar 17, 2015·Structure·Natalie K GarciaKelly K Lee
Oct 29, 2013·Biochimica Et Biophysica Acta·Caroline M MairChristian Sieben
Jun 9, 2016·Biophysical Journal·Santiago Di LellaCaroline M Mair
Dec 7, 2017·International Journal of Molecular Sciences·Rodion J MolotkovskySergey A Akimov
Feb 20, 2010·The EMBO Journal·Kelly K Lee
Apr 3, 2003·Nature Reviews. Molecular Cell Biology·Peter M Colman, Michael C Lawrence
Nov 5, 2003·Nature Structural Biology·Heather E ParkJudith M White
Apr 21, 2005·Microbiology and Immunology·Makoto UjikeEri Nobusawa
Mar 8, 2006·The Biochemical Journal·Ding-Kwo Chang, Shu-Fang Cheng
Jan 16, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Sivaramakrishna RachakondaAndreas Herrmann
Apr 24, 2018·Trends in Microbiology·Charles J RussellFaten A Okda
Jul 23, 2003·Biochimica Et Biophysica Acta·José L Nieva, Aitziber Agirre
Sep 14, 2004·Biomacromolecules·Jason N CampbellPaul S Russo
Nov 17, 2021·Biochemical Society Transactions·Alzbeta Tuerkova, Peter M Kasson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.