Dec 15, 2015

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

BioTechniques
Nathan WalesM Thomas P Gilbert

Abstract

An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained <3% endogenous DNA, but this enrichment is less pronounced when dsDNA preparations successfully recover short endogenous DNA fragments (mean size < 70 bp). Our findings can help researchers determine when to utilize the time- and resource-intensive ssDNA library preparation method.

  • References12
  • Citations6

References

Mentioned in this Paper

AdnA protein, Pseudomonas fluorescens
Research Personnel
Ancient DNA
Sequence Determinations, DNA
probe gene fragment
cDNA Library
Nucleic Acid Strand
DNA
Bowel Preparation
DNA, Single-Stranded

Related Feeds

Ancient DNA

Ancient DNA sequences are able to offer valuable insights into molecular evolutionary processes, but are notoriously difficult to analyze due to molecular damage and exogenous dna contamination. Discover the latest research on Ancient DNA here.

© 2020 Meta ULC. All rights reserved