New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA
BioTechniques
Dec 15, 2015
Nathan WalesM Thomas P Gilbert
PMID: 26651516
DOI: 10.2144/000114364
Abstract
An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and...read more
Mentioned in this Paper
AdnA protein, Pseudomonas fluorescens
Research Personnel
Ancient DNA
Sequence Determinations, DNA
probe gene fragment
cDNA Library
Nucleic Acid Strand
DNA
Bowel Preparation
DNA, Single-Stranded
1
79
1
Paper Details
References
- References12
- Citations6
Pulling out the 1%: whole-genome capture for the targeted enrichment of ancient DNA sequencing libraries
American Journal of Human GeneticsFebruary 27, 2014Meredith L CarpenterCarlos D Bustamante1725251
Complete mitochondrial genome sequence of a Middle Pleistocene cave bear reconstructed from ultrashort DNA fragments
Proceedings of the National Academy of Sciences of the United States of AmericaSeptember 11, 2013Jesse DabneyMatthias Meyer537859911
A high-coverage genome sequence from an archaic Denisovan individual
ScienceSeptember 1, 2012Matthias MeyerSvante Pääbo103399138514Library construction for ancient genomics: single strand or double strand?
BioTechniquesJune 14, 2014E Andrew BennettThierry Grange61151
Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA
Nature ProtocolsMarch 16, 2013Marie-Theres Gansauge, Matthias Meyer12525
Reconstructing ancient genomes and epigenomes
Nature Reviews. GeneticsJune 10, 2015Ludovic OrlandoEske Willerslev81116491
Predicting the molecular complexity of sequencing libraries
Nature MethodsFebruary 26, 2013Timothy Daley, Andrew D Smith3022462
Selective enrichment of damaged DNA molecules for ancient genome sequencing
Genome ResearchAugust 2, 2014Marie-Theres Gansauge, Matthias Meyer621271
A mitochondrial genome sequence of a hominin from Sima de los Huesos
NatureDecember 7, 2013Matthias MeyerSvante Pääbo324724174742Reduced fitness in progeny from old parents in a natural population
Proceedings of the National Academy of Sciences of the United States of AmericaMarch 17, 2015Julia SchroederTerry Burke6233142
12
- References12
- Citations6
Extraction of ultrashort DNA molecules from herbarium specimens
BioTechniquesFebruary 15, 2017Rafal M GutakerHernán A Burbano5163
Novel Substrates as Sources of Ancient DNA: Prospects and Hurdles
GenesJuly 14, 2017Eleanor Joan Green, Camilla F Speller2381
Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
Scientific ReportsDecember 24, 2017Ana Y Morales-ArceChristina Warinner658482
Draft genome of the fungus-growing termite pathogenic fungus Ophiocordyceps bispora (Ophiocordycipitaceae, Hypocreales, Ascomycota)
Data in BriefMarch 30, 2017Benjamin H ConlonHenrik H de Fine Licht5116Recent Asian origin of chytrid fungi causing global amphibian declines
ScienceMay 12, 2018Simon J O'HanlonMatthew C Fisher1032892937411
Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase
Nucleic Acids ResearchJanuary 26, 2017Marie-Theres GansaugeMatthias Meyer18185
1
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New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA
Abstract
An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and...read more
Mentioned in this Paper
AdnA protein, Pseudomonas fluorescens
Research Personnel
Ancient DNA
Sequence Determinations, DNA
probe gene fragment
cDNA Library
Nucleic Acid Strand
DNA
Bowel Preparation
DNA, Single-Stranded
1
79
1
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A simple fluorometric assay for DNA exonuclease activity based on graphene oxide
The AnalystMarch 14, 2012
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Paper Details
References
- References12
- Citations6
Pulling out the 1%: whole-genome capture for the targeted enrichment of ancient DNA sequencing libraries
American Journal of Human GeneticsFebruary 27, 2014Meredith L CarpenterCarlos D Bustamante1725251Complete mitochondrial genome sequence of a Middle Pleistocene cave bear reconstructed from ultrashort DNA fragments
Proceedings of the National Academy of Sciences of the United States of AmericaSeptember 11, 2013Jesse DabneyMatthias Meyer537859911A high-coverage genome sequence from an archaic Denisovan individual
ScienceSeptember 1, 2012Matthias MeyerSvante Pääbo103399138514Library construction for ancient genomics: single strand or double strand?
BioTechniquesJune 14, 2014E Andrew BennettThierry Grange61151Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA
Nature ProtocolsMarch 16, 2013Marie-Theres Gansauge, Matthias Meyer12525Reconstructing ancient genomes and epigenomes
Nature Reviews. GeneticsJune 10, 2015Ludovic OrlandoEske Willerslev81116491Predicting the molecular complexity of sequencing libraries
Nature MethodsFebruary 26, 2013Timothy Daley, Andrew D Smith3022462Selective enrichment of damaged DNA molecules for ancient genome sequencing
Genome ResearchAugust 2, 2014Marie-Theres Gansauge, Matthias Meyer621271A mitochondrial genome sequence of a hominin from Sima de los Huesos
NatureDecember 7, 2013Matthias MeyerSvante Pääbo324724174742Reduced fitness in progeny from old parents in a natural population
Proceedings of the National Academy of Sciences of the United States of AmericaMarch 17, 2015Julia SchroederTerry Burke6233142
12
- References12
- Citations6
Extraction of ultrashort DNA molecules from herbarium specimens
BioTechniquesFebruary 15, 2017Rafal M GutakerHernán A Burbano5163Novel Substrates as Sources of Ancient DNA: Prospects and Hurdles
GenesJuly 14, 2017Eleanor Joan Green, Camilla F Speller2381Successful reconstruction of whole mitochondrial genomes from ancient Central America and Mexico
Scientific ReportsDecember 24, 2017Ana Y Morales-ArceChristina Warinner658482Draft genome of the fungus-growing termite pathogenic fungus Ophiocordyceps bispora (Ophiocordycipitaceae, Hypocreales, Ascomycota)
Data in BriefMarch 30, 2017Benjamin H ConlonHenrik H de Fine Licht5116Recent Asian origin of chytrid fungi causing global amphibian declines
ScienceMay 12, 2018Simon J O'HanlonMatthew C Fisher1032892937411Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase
Nucleic Acids ResearchJanuary 26, 2017Marie-Theres GansaugeMatthias Meyer18185
1