New interaction partners for Nek4.1 and Nek4.2 isoforms: from the DNA damage response to RNA splicing

Proteome Science
Fernanda Luisa BaseiJörg Kobarg

Abstract

Neks are serine-threonine kinases that are similar to NIMA, a protein found in Aspergillus nidulans which is essential for cell division. In humans there are eleven Neks which are involved in different biological functions besides the cell cycle control. Nek4 is one of the largest members of the Nek family and has been related to the primary cilia formation and in DNA damage response. However, its substrates and interaction partners are still unknown. In an attempt to better understand the role of Nek4, we performed an interactomics study to find new biological processes in which Nek4 is involved. We also described a novel Nek4 isoform which lacks a region of 46 amino acids derived from an insertion of an Alu sequence and showed the interactomics profile of these two Nek4 proteins. Isoform 1 and isoform 2 of Nek4 were expressed in human cells and after an immunoprecipitation followed by mass spectrometry, 474 interacting proteins were identified for isoform 1 and 149 for isoform 2 of Nek4. About 68% of isoform 2 potential interactors (102 proteins) are common between the two Nek4 isoforms. Our results reinforce Nek4 involvement in the DNA damage response, cilia maintenance and microtubule stabilization, and raise the possibilit...Continue Reading

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Citations

Apr 27, 2017·Plant & Cell Physiology·Wen-Jia PanJin-Song Zhang
Feb 10, 2019·International Journal of Cancer. Journal International Du Cancer·Vibha SinghArrigo De Benedetti
May 6, 2020·Proteome Science·Andressa Peres de OliveiraJörg Kobarg
Apr 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andressa Peres de OliveiraJörg Kobarg
Aug 7, 2019·Cellular and Molecular Neurobiology·Benedetta PerroneSebastiano Cavallaro
Mar 7, 2021·Cells·Isadora Carolina Betim PavanJörg Kobarg
Jul 17, 2021·SLAS Discovery·Mariafrancesca ScaliseCesare Indiveri

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Datasets Mentioned

BETA
KJ592714

Methods Mentioned

BETA
immunoprecipitation
X-ray
GTPase
pull-down
Confocal microscopy
PCR
FCS
transfection
PCRs

Software Mentioned

ScaffoldQ +
MS
IP
Mascot
Volocity
NucPred
imageJ
Proteome
FIJI
Mascot engine

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