New Intracellular Shikimic Acid Biosensor for Monitoring Shikimate Synthesis in Corynebacterium glutamicum

ACS Synthetic Biology
Chang LiuShuang-Jiang Liu

Abstract

The quantitative monitoring of intracellular metabolites with in vivo biosensors provides an efficient means of identifying high-yield strains and observing product accumulation in real time. In this study, a shikimic acid (SA) biosensor was constructed from a LysR-type transcriptional regulator (ShiR) of Corynebacterium glutamicum. The SA biosensor specifically responded to the increase of intracellular SA concentration over a linear range of 19.5 ± 3.6 to 120.9 ± 1.2 fmole at the single-cell level. This new SA biosensor was successfully used to (1) monitor the SA production of different C. glutamicum strains; (2) develop a novel result-oriented high-throughput ribosome binding site screening and sorting strategy that was used for engineering high-yield shikimate-producing strains; and (3) engineer a whole-cell biosensor through the coexpression of the SA sensor and a shikimate transporter shiA gene in C. glutamicum RES167. This work demonstrated that a new intracellular SA biosensor is a valuable tool facilitating the fast development of microbial SA producer.

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Citations

Mar 30, 2018·Applied Microbiology and Biotechnology·Xiaoyuan WangPeter J Quinn
Mar 31, 2019·Biotechnology Journal·Roberto G StellaJulia Frunzke
Mar 7, 2020·Frontiers in Bioengineering and Biotechnology·Jia-Wei LiYun-Peng Bai
Aug 7, 2018·BioMed Research International·Ulysses Amancio de FriasRafael Silva-Rocha
Aug 5, 2021·Journal of Industrial Microbiology & Biotechnology·Jennifer A Kaczmarek, Kristala L J Prather
Apr 4, 2019·Metabolic Engineering·Volker F Wendisch

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