New Isoflavonoids from the extract of Rhynchosia precatoria (Humb. & Bonpl. ex Willd.) DC. and their antimycobacterial activity

Journal of Ethnopharmacology
Enrique Wenceslao Coronado-AcevesMaria Carla Marcotullio

Abstract

The evaluation of the antimycobacterial activity of extracts of medicinal plants used by Mayos against tuberculosis and respiratory problems, allowed the identification of Rhynchosia precatoria (Humb. & Bonpl. ex Willd.) DC (Fabaceae) as the best candidate to find new antimycobacterial compounds. To isolate and characterize the compounds of R. precatoria responsible for the inhibitory and bactericidal activity against Mycobacterium tuberculosis H37Rv and Mycobacterium smegmatis ATCC 700084. To determine antimycobacterial synergistic effect of pure compounds and their selectivity index towards Vero cells. A total of six flavonoids were purified by silica gel column chromatography. Structural elucidation of the isolated compounds was achieved by using 1D and 2D NMR spectroscopy techniques. The configuration at the C-3 chiral center was established by quantum mechanical calculation of the electronic circular dichroism (ECD) spectrum. In vitro inhibitory and bactericidal activity against M. tuberculosis and M. smegmatis were determined with the redox indicator Alamar Blue (resazurin). Synergy was determined by X/Y quotient. Cytotoxicity was measured by MTT assay. The isolated compounds were identified as precatorin A (1), precatori...Continue Reading

References

Jan 5, 2001·Phytochemistry·A YenesewM G Peter
Aug 12, 2005·Chemical Reviews·Jacopo TomasiRoberto Cammi
Oct 8, 2005·Attachment & Human Development·Arietta SladeAlison Locker

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Citations

Jul 4, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Malose J MphahleleYee Siew Choong
Jan 12, 2019·Natural Product Reports·Nawaf Al-Maharik
Mar 13, 2019·International Journal of Mycobacteriology·Beverley Cherie Millar, John Edmund Moore
Jul 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhamad Harith MazlunZunoliza Abdullah
Oct 20, 2020·Journal of Natural Products·Roukia ZatoutAntonio Evidente

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