New lipase assay using Pomegranate oil coating in microtiter plates

Biochimie
Serdar ÜlkerJean-François Cavalier

Abstract

Lipases play various roles in fat digestion, lipoprotein metabolism, and in the mobilization of fat stored in lipid bodies in animals, plants and microorganisms. In association with these physiological functions, there is an important field of research for discovering lipase inhibitors and developing new treatments of diseases such as obesity, atherosclerosis, diabetes and tuberculosis. In this context, the development of convenient, specific and sensitive analytical methods for the detection and assay of lipases and/or lipase inhibitors is of major importance. It is shown here that purified triacylglycerols (TAGs) from Punica granatum (Pomegranate) seed oil coated on microtiter plates can be used for the continuous assay of lipase activity by recording the variations with time of the UV absorption spectra at 275 nm. UV absorption is due the release of punicic acid (9Z,11E,13Z-octadeca-9,11,13-trienoic acid), a conjugated triene contained in Pomegranate oil. This new microtiter plate assay allows to accurately measure the activity of a wider range of lipases compared to the similar assay previously developed with Tung oil containing α-eleostearic acid (9Z,11E,13E-octadeca-9,11,13-trienoic acid), including the LipY lipase from M...Continue Reading

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Citations

Nov 30, 2018·Bioscience Reports·Pierre SantucciStéphane Canaan
Dec 12, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Maria Angeles Camacho-RuizJorge A Rodríguez
Oct 20, 2020·European Journal of Medicinal Chemistry·Jean-François CavalierStéphane Canaan
Jan 14, 2021·Biotechnology and Applied Biochemistry·Surabhi Soni

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