New metallothionein assay in Scrobicularia plana: heating effect and correlation with other biomarkers

Environmental Pollution
Antonio Romero-RuizJuan López-Barea

Abstract

Metallothionein (MT) and other biomarker levels were measured in Scrobicularia plana clams to assess pollution of the Guadalquivir Estuary possibly affected by metals released from Aznalcóllar pyrite mine in 1998. After optimizing reagent concentrations for monobromobimane derivatization, MT levels were quantified by reversed-phase high-performance liquid chromatography coupled to fluorescence detection (RP-HPLC-FD) in heated or unheated digestive gland extracts and compared to those obtained by differential pulse polarography (DPP). MT content assayed by RP-HPLC-FD in unheated samples was higher than that obtained by DPP and correlated better with metals and anti-oxidant activities. MT assay by RP-HPLC-FD in unheated extracts would be preferable for assessing metal pollution, due to its greater sensitivity and specificity. In addition to MT induction, glyoxalase II inhibition was well correlated with metal contents. Our results suggest that metals at the estuary do not originate from Aznalcóllar spill, but from those carried along by the river and deposited at its concave bank.

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Citations

Jul 8, 2010·Biological Trace Element Research·Fatemeh Shariati, Shahab Shariati
Mar 19, 2014·Journal of Proteomics·Miguel Ángel García-SevillanoJosé Luis Gómez-Ariza
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Jul 23, 2014·Marine Pollution Bulletin·Victoria TorneroJulián Blasco
Jun 13, 2014·Environmental Science & Technology·M A García-SevillanoJ L Gómez-Ariza

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