New method for counting bacteria associated with coral mucus.

Applied and Environmental Microbiology
Melissa Garren, Farooq Azam

Abstract

The ability to count bacteria associated with reef-building corals in a rapid, reliable, and cost-effective manner has been hindered by the viscous and highly autofluorescent nature of the coral mucus layer (CML) in which they live. We present a new method that disperses bacterial cells by trypsinization prior to 4',6-diamidino-2-phenylindole (DAPI) staining and quantification by epifluorescence microscopy. We sampled seawater and coral mucus from Porites lobata from 6 reef sites influenced by wastewater intrusion and 2 reef sites unaffected by wastewater in Hawaii. Bacterial and zooxanthella abundances and cell sizes were quantified for each sample. Bacteria were more abundant in coral mucus (ranging from 5.3 x 10(5) +/- 1.0 x 10(5) cells ml(-1) to 1.8 x 10(6) +/- 0.2 x 10(6) cells ml(-1)) than in the surrounding seawater (1.9 x 10(5) +/- 0.1 x 10(5) cells ml(-1) to 4.2 x 10(5) +/- 0.2 x 10(5) cells ml(-1)), and the mucus-associated cells were significantly smaller than their seawater counterparts at all sites (P < 0.0001). The difference in cell size between mucus- and seawater-associated bacteria decreased at wastewater-influenced sites, where simultaneously mucus bacteria were larger and seawater bacteria were smaller than ...Continue Reading

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Citations

Dec 23, 2011·The ISME Journal·Melissa Garren, Farooq Azam
Feb 1, 2013·Proceedings. Biological Sciences·Cory J KredietMax Teplitski
Jun 26, 2012·Applied and Environmental Microbiology·Amandine LerusteYvan Bettarel
Sep 29, 2015·Molecular Ecology·Linda L BlackallMadeleine J H van Oppen
Oct 1, 2011·Environmental Microbiology·Melissa Garren, Farooq Azam
Jun 16, 2016·Journal of Microbiological Methods·Yvan BettarelBouvier Thierry
May 16, 2018·Microbes and Environments·Jia-Ho ShiuSen-Lin Tang
Jun 21, 2015·Applied and Environmental Microbiology·Hanh Nguyen-KimJustine Brune
Jan 18, 2015·Applied and Environmental Microbiology·Cátia CarreiraCorina P D Brussaard
Jul 3, 2019·Nature Reviews. Microbiology·Madeleine J H van Oppen, Linda L Blackall
Jul 29, 2018·Scientific Reports·Yvan BettarelJean-Dominique Durand
Jul 20, 2017·NPJ Biofilms and Microbiomes·Cynthia B Silveira, Forest L Rohwer
Jul 1, 2020·Frontiers in Microbiology·Mark McCauleyTamar L Goulet
Nov 4, 2020·Microorganisms·Aurélie BoilardNicolas Derome
Apr 4, 2021·International Journal of Molecular Sciences·Hongfei SuFen Wei
May 23, 2021·BMC Microbiology·Justin MaireMadeleine J H van Oppen

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