New Method for isolation of immunologically pure pili from Escherichia coli.

Infection and Immunity
T K KorhonenC S Edén

Abstract

A new technique for purification of bacterial pili was developed and applied to Escherichia coli strains isolated from the urine of patients with symptomatic urinary tract infections. After mechanical detachment from the bacterial cells, the pili were concentrated by precipitation with ammonium sulfate, dialyzed, and solubilized in buffer containing deoxycholate. The fraction containing the pili was purufied further by ultracentrifugation in a sucrose gradient and by elution through a Sepharose 4B column in 6 M urea buffer. The pilus filaments were not dissociated by concentrated urea and were eluted in the void volume of the column. The purified pili had a molecular weight of 17,000. The isoelectric point of the pili from one of the strains was 4.9, and about 43% of the amino acids were hydrophobic. Hyperimmunesera raised in rabbits against the purified pili did not contain detectable antibodies to the lipopolysaccharide O antigen or to the capsular polysaccharide K antigen of the homologous strain. The pili obtained by this purification procedure are free from other detectable bacterial surface antigens, and the purified pilus filaments are of relatively homogeneous size. This procedure enables purification of the pili also f...Continue Reading

References

Nov 1, 1977·The Journal of Experimental Medicine·I E Salit, E C Gotschlich
Jun 1, 1979·Journal of Bacteriology·J C McMichael, J T Ou
Nov 1, 1977·The Journal of Experimental Medicine·I E Salit, E C Gotschlich
Jan 15, 1979·Analytical Biochemistry·M Saraste, T K Korhonen
Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Jan 1, 1975·Annual Review of Microbiology·J C Ottow
Aug 1, 1973·Infection and Immunity·A P Punsalang, W D Sawyer
Jan 1, 1971·Journal of Bacteriology·M L DePamphilis, J Adler
Aug 1, 1959·Journal of General Microbiology·J P DUGUID
Mar 1, 1950·Biochimica Et Biophysica Acta·A L HOUWINK, W van ITERSON

❮ Previous
Next ❯

Citations

Jan 1, 1986·Microbiology and Immunology·T YanoA F Pestana de Castro
Sep 18, 2004·Journal of Bacteriology·Puneet KhandelwalNirupama Banerjee
Feb 8, 2006·Applied and Environmental Microbiology·Olivia GohlBeate Averhoff
Aug 29, 2014·Physical Chemistry Chemical Physics : PCCP·Yan-Ling ZhaoMichel A Van Hove
Aug 1, 1990·Molecular Microbiology·S J HultgrenS K Amundsen
Jan 1, 1994·Microbiology and Immunology·P MuscasG Satta
Apr 1, 1983·Australian Veterinary Journal·K J FaheyB L Clark
Jan 1, 1983·Critical Reviews in Microbiology·G W Jones, R E Isaacson
Oct 1, 1989·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M B KaackJ A Roberts
Sep 5, 2006·Journal of Bacteriology·Jyotirmoy BanerjeeNirupama Banerjee
Jan 11, 2008·Clinical and Vaccine Immunology : CVI·Anu KanteleJussi M Kantele
Sep 25, 2009·Water Environment Research : a Research Publication of the Water Environment Federation·Chul Park, John T Novak
Aug 1, 1990·Molecular Microbiology·A M TarkkanenT K Korhonen
Jun 1, 1994·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·H MiyataS Maki
Jul 3, 1989·FEBS Letters·J Parkkinen, T K Korhonen
Feb 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N AgataN Kato
Jan 1, 1990·The Journal of Urology·J A RobertsG Baskin
Aug 16, 2018·Frontiers in Microbiology·Wichuda PhothichaisriSurang Chankhamhaengdecha
Jun 1, 1983·The Journal of Urology·J A Roberts
Nov 1, 1980·Journal of Bacteriology·T K KorhonenN Kuusi
Oct 1, 1981·Journal of Bacteriology·Y EshdatN Sharon
Oct 1, 1984·Journal of Bacteriology·D C Dodd, B I Eisenstein
Nov 1, 1984·Journal of Bacteriology·B NowickiT K Korhonen
Dec 1, 1987·Infection and Immunity·B NowickiS I Hull
Mar 1, 1990·Infection and Immunity·M Dho-MoulinJ P Lafont

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.