PMID: 591723Jan 1, 1977

New method for obtaining IgA-specific protease

Journal of Immunological Methods
T B HigerdJ W Fett

Abstract

A simple method for obtaining an active preparation of IgA-specific protease from a bacterial source is presented. In this method Streptococcus sanguis was inoculated onto the surface of a dialysis membrane on nutrient agar. Following growth, the membrane was removed from the agar surface and washed in a small volume of buffer. A solution with protease activity against IgA1 monoclonal proteins was obtained by clarification of the wash and appeared to be similar to enzyme preparations obtained by other methods.

References

Aug 1, 1975·British Journal of Haematology·G VirellaF Graça

Citations

Sep 1, 1983·Molecular Immunology·M KilianH Bleeg
Oct 28, 1983·Journal of Immunological Methods·J Reinholdt, M Kilian
Aug 1, 1981·Bulletin of Environmental Contamination and Toxicology·R W FerenbaughD M LaCombe

Related Concepts

Antibody Specificity
SDS-PAGE
IgA2
Immunoelectrophoresis
Peptide Hydrolases
Streptococcus sanguis

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