New methods of assessing crystal growth and saturation of brushite in whole urine: effect of pH, calcium and citrate.

The Journal of Urology
Charles Y C PakK Sakhaee

Abstract

Brushite crystallization might be important in stone formation and prevention. To explore this question new methods for the saturation and crystal growth of brushite were devised that are applicable to whole urine without any computer program. The saturation value (concentration-to-product ratio) was determined by dividing the molar concentration product of Ca ([Ca]) and phosphate ([P]), that is [Ca] x [P], of original urine by the steady state solubility obtained after incubating with an excess of brushite (10 mg/ml) for 5 hours. Crystal growth was measured from the depletion of filtrate ([Ca] x [P]) 3 hours after seeding with brushite (0.25 mg/ml). To test the effect of pH, Ca and citrate the saturation value and crystal growth were determined in 24-hour urine samples from 4 normal volunteers and 2 stone formers, and modified artificially to produce 4 ranges of pH, Ca and citrate by adding acid, base, Ca or citrate. The saturation value and crystal growth of brushite increased with an increase in pH or the Ca concentration but they decreased when the citrate concentration increased. The saturation value correlated strongly with crystal growth. The new methods of brushite saturation value and crystal growth should help discern...Continue Reading

References

Jun 1, 1976·Metabolism: Clinical and Experimental·C Y Pak, K Holt
Dec 1, 1985·The Journal of Urology·P G WernessB Finlayson
Nov 1, 1985·The Journal of Urology·C Y PakJ Harvey
Jun 1, 1993·The Journal of Urology·C L Wabner, C Y Pak
Mar 6, 2003·The Journal of Clinical Investigation·Andrew P EvanMarc Grynpas
Jul 16, 2004·Kidney International·Joan H ParksJames E Lingeman
Oct 27, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Allen RodgersGraham Jackson
Sep 26, 2006·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Brian J WelchKhashayar Sakhaee

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