New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.).

3 Biotech
Khaled ElmeerAladdin Hamwieh

Abstract

New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and the remaining ten primers (33.4%) generated polymorphic banding patterns. A total of 77 alleles have been observed with a mean of 7.7 alleles per locus. The average of gene diversity was 0.80 ranging from 0.6 (in marker DP168) to 0.9 (in two markers DP157 and DP175). These new co-dominant markers will be a starting point for researchers making use of the markers for genetic mapping and diversity analysis of date palm.

References

Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Feb 1, 1997·Genome Génome / Conseil National De Recherches Canada·B Vosman, P Arens
Jun 26, 1999·Genome Génome / Conseil National De Recherches Canada·T Areshchenkova, M W Ganal
Jul 25, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N S Al-Khalifah, E Askari
Feb 12, 2005·Bioinformatics·Kejun Liu, Spencer V Muse

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Citations

Dec 7, 2017·Frontiers in Plant Science·Zilong HeSongnian Hu

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Methods Mentioned

BETA
PCR

Software Mentioned

PowerMarker
Alpha View
TreeView

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