New microsatellite markers for Campanula scheuchzeri (Campanulaceae), with cross-amplification in C. rotundifolia

Applications in Plant Sciences
Georg F J Armbruster, Jürg Stöcklin

Abstract

We developed new microsatellite primers for the alpine bellflower Campanula scheuchzeri. Allelic polymorphisms will be used to study differentiation along elevation gradients of C. scheuchzeri populations and in the co-occurring sister-species C. rotundifolia in the Alps. We analyzed C. scheuchzeri from three high-elevation sites and C. rotundifolia from two low-elevation sites in Switzerland. Campanula scheuchzeri was found to be tetraploid (2n = 68 = 4x), and up to 22 alleles were found per locus and population. Of the 15 polymorphic loci developed for C. scheuchzeri, 10 loci were tested, all of which amplified in C. rotundifolia, with similar amplicon length. Campanula rotundifolia individuals also showed tetraploid signals. We speculate that C. scheuchzeri and C. rotundifolia share a common gene pool and evolve under vicariance. This presents a testable hypothesis that will be evaluated through future work. Our developed primers might also amplify in other related Campanula taxa.

References

Feb 5, 2000·Nature Biotechnology·M Schuelke
Feb 10, 2007·Clinical Chemistry·Claudia LangebrakeDirk Reinhardt
Sep 10, 2014·Applications in Plant Sciences·Halil KesselringGeorg F J Armbruster
Sep 10, 2014·Applications in Plant Sciences·Elena HamannGeorg F J Armbruster

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Datasets Mentioned

BETA
EF088759
EF088762

Methods Mentioned

BETA
PCR

Software Mentioned

ATETRA

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