New model substrates for enzymes hydrolysing polyethyleneterephthalate and polyamide fibres

Journal of Biochemical and Biophysical Methods
Sonja HeumannGeorg M Gübitz

Abstract

Recently the potential of enzymes for surface hydrophilisation and/or functionalisation of polyethyleneterephthalate (PET) and polyamide (PA) has been discovered. However, there was no correlation between enzyme class/activity (e.g. esterase, lipase, cutinase) and surface hydrolysis of these polymers and consequently no simple assay to estimate this capability. Enzymes active on the model substrates bis (benzoyloxyethyl) terephthalate and adipic acid bishexyl-amide, were also capable of increasing the hydrophilicity of PET and PA. When dosed at the identical activity on 4-nitrophenyl butyrate, only enzymes from Thermobifida fusca, Aspergillus sp., Beauveria sp. and commercial enzymes (TEXAZYME PES sp5 and Lipase PS) increased the hydrophilicity of PET fibres while other esterases and lipases did not show any effect. Activity on PET correlated with the activity on the model substrate. Hydrophilicity of fibres was greatly improved based on increases in rising height of up to 4.3 cm and the relative decrease of water absorption time between control and sample of the water was up to 76%. Similarly, enzymes increasing the hydrophilicity of PA fibres such as from Nocardia sp., Beauveria sp. and F. solani hydrolysed the model substrat...Continue Reading

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Citations

Feb 23, 2010·Applied Microbiology and Biotechnology·Nitat SinsereekulVerawat Champreda
May 15, 2010·Applied Microbiology and Biotechnology·Susan BilligWolfgang Zimmermann
Apr 23, 2013·Applied Biochemistry and Biotechnology·Krishnamoorthy Hegde, Venkata Dasu Veeranki
Jul 24, 2013·Preparative Biochemistry & Biotechnology·Mazeyar Parvinzadeh GashtiMahyar Parvinzadeh Gashti
May 24, 2014·Genome Announcements·Elena P IvanovaTomoo Sawabe
Jun 26, 2014·Applied Microbiology and Biotechnology·Keisuke NagaiSeiji Negoro
Feb 16, 2016·Environmental Science & Technology·Veronika PerzGeorg M Guebitz
Nov 27, 2007·Trends in Biotechnology·Georg M Guebitz, Artur Cavaco-Paulo
Oct 23, 2008·Biotechnology and Bioengineering·Sonja HeumannGeorg M Guebitz
May 17, 2011·Biotechnology Progress·Doris RibitschGeorg M Guebitz
Nov 3, 2015·Biotechnology and Bioengineering·Veronika PerzGeorg M Guebitz
May 22, 2013·BioMed Research International·Divya PrakashBalasaheb Kapadnis
Sep 24, 2013·Biotechnology Advances·Sheng ChenJing Wu
Apr 15, 2011·Experimental Dermatology·Andrea HasmannEva Wehrschuetz-Sigl
Nov 10, 2017·Chembiochem : a European Journal of Chemical Biology·Karolina HaernvallGeorg M Guebitz
Jun 9, 2020·Applied Biochemistry and Biotechnology·Nathania PuspitasariCheng-Kang Lee
Apr 18, 2013·Biotechnology and Bioengineering·Enrique Herrero AceroGeorg M Guebitz
Jan 21, 2021·Chembiochem : a European Journal of Chemical Biology·Alessandro BerselliMaria Cristina Menziani

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