New monoclonal antibody-based test for Helicobacter pylori urease in gastric tissue

The Korean Journal of Internal Medicine
Do Hyun KimYoung Do Jung

Abstract

To evaluate a new monoclonal antibody for Helicobacter pylori urease in gastric tissue. A total of 107 volunteers were enrolled. All subjects underwent a (13)C-urea breath test and esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia. Six biopsy specimens in the gastric antrum and body were obtained for a rapid urease test and histology. The new monoclonal antibody-based H. pylori urease test (HPU) was performed to rapidly and qualitatively detect urease in two biopsy specimens. H. pylori infection was diagnosed in 73 subjects. The sensitivity and specificity of the HPU was 89% and 74%, respectively. The subjects were divided into two groups: one with true-positive and true-negative HPU results (n = 90) and the other with false-positive and false-negative HPU results (n = 17). Across all subjects, ammonia levels were 900.5 ± 646.7 and 604.3 ± 594.3 μmol/L (p > 0.05), and pH was 3.37 ± 1.64 and 2.82 ± 1.51 (p > 0.05). Sensitivity was higher in the presence of atrophic gastritis or intestinal metaplasia. HPU detected H. pylori in approximately 10 min. Gastric aspirate ammonia and pH levels did not affect the test results. Sensitivity was good in the presence of atrophic gastritis or intestinal metaplasia.

References

Apr 1, 1987·Journal of Clinical Pathology·M BorromeoK J Pinkard
May 1, 1994·Gastrointestinal Endoscopy·H M HackG Triadafilopoulos
Jan 25, 1997·Lancet·C S GoodwinT C Northfield
Nov 24, 1999·European Journal of Gastroenterology & Hepatology·M I PrinceJ R Barton
Aug 2, 2000·Journal of Gastroenterology and Hepatology·Y TokunagaK Ohsumi
Apr 20, 2001·Journal of Microbiological Methods·L MonteiroF Mégraud
Sep 15, 2001·Zeitschrift für Gastroenterologie·K Agha-AmiriP Malfertheiner
Dec 1, 2004·Alimentary Pharmacology & Therapeutics·J P Gisbert, J M Pajares

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Citations

Mar 16, 2018·The Cochrane Database of Systematic Reviews·Lawrence Mj BestKurinchi Selvan Gurusamy
Aug 18, 2016·Helicobacter·Francis MégraudPhilippe Lehours

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Methods Mentioned

BETA
biopsy
infrared spectrometry
biopsies

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Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.