New multispecific array as a tool for electrochemical impedance spectroscopy-based biosensing

Biosensors & Bioelectronics
E KomarovaA Bogomolova

Abstract

Using electrochemical impedance spectroscopy (EIS) for biosensing applications typically requires repetitive experiments. To address this need, we have designed a multispecific electrochemical array with eight individually addressable 2mm-diameter gold working electrodes for rapid biosensing data accumulation by EIS in the presence of redox agent. The array allows to incorporate multiple negative controls in the course of a single binding experiment, as well as to perform parallel identical experiments to improve reliability of detection. The array is fit with attached electrochemical cell with Ag/AgCl mini reference electrode and can be used to process macro samples of 0.5-1 ml or micro samples of 5 microl in a drop-wise fashion. Eight individual EIS measurements are completed in 15 min. The reported array is disposable, economical and is easy to use. Examples of array use for label-free genetic sensing of 2.7 kb-long target Yersinia pestis DNA and for protein sensing of Ricin Toxin Chain A (RTA) are presented. We suggest the reported array design as a tool for researchers in the area of EIS sensing.

References

Oct 11, 2005·Methods : a Companion to Methods in Enzymology·Vincent GauDavid A Haake
Jan 31, 2006·IEE Proceedings. Nanobiotechnology·R Moeller, W Fritzsche
Sep 27, 2006·Nucleic Acids Research·Siu-Wai YeungI-Ming Hsing
Jan 10, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Elizabeth PavlovicH T Soh
Feb 12, 2008·Analytica Chimica Acta·Fausto LucarelliMarco Mascini
Apr 9, 2009·Chembiochem : a European Journal of Chemical Biology·Sebastian NeugebauerGerhard Hartwich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.