New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering

Materials
Maria RâpăRuxandra Vidu

Abstract

Concentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG), and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with the energy dispersive X-ray spectroscopy (SEM/EDS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), and indentation tests. The cytotoxicity of the electrospun nanofibers was conducted on L929 fibroblast cells using MTT and LDH assays and cell morphology observations. The electrospun RCG and KH nanofibers morphology showed an average size of nanofibers ranging between 44 and 410 nm, while the electrospun HC10CC nanofibers exhibited higher sizes. The ATR-FTIR spectra performed both on extracted proteins and electrospun nanofibers showed that the triple helix structure of collagen is partially preserved. The results were in agreement with the circular dichroism analysis for protein extracts. Furthermore, the viscoelastic properties of electrospun KH nanofibers were superior to those of electrospun RCG nanofibers. Based on both in vitro quantitative and qualitativ...Continue Reading

References

Nov 19, 2003·Advanced Drug Delivery Reviews·Zbigniew Ruszczak, Wolfgang Friess
Mar 4, 2008·Biomaterials·Dimitrios I ZeugolisMichael Raghunath
Jul 5, 2011·Biophysical Journal·Laurent Bozec, Marianne Odlyha
Apr 9, 2013·Nanomedicine·Wan Ting SowKee Woei Ng
Apr 24, 2013·International Journal of Biological Macromolecules·Sudheesh K ShuklaBhekie B Mamba
Aug 9, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Davide BelliniPietro Matricardi
Sep 16, 2016·Scientific Reports·Alexandre G de Brevern
Aug 16, 2017·International Journal of Pharmaceutics·Ivan J Hall BarrientosDimitrios A Lamprou
Aug 16, 2017·International Journal of Biological Macromolecules·Jing WangDan Zhou
Sep 29, 2017·Biochemistry and Biophysics Reports·Jayalakshmi KrishnamoorthiAnnaian Shanmugam
Dec 31, 2018·International Journal of Biological Macromolecules·Deqiang Wang, Weifeng Jiang
Nov 12, 2019·Journal of Biomaterials Science. Polymer Edition·Shujuan GuoYi Ding
Jun 24, 2020·International Journal of Biological Macromolecules·Abbas Zakeri BazmandehYounes Ghasemi
Oct 1, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Adrian D Juncos BombinHelen O McCarthy

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Citations

Apr 4, 2021·Bioengineering·Britani N BlackstoneHeather M Powell

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Methods Mentioned

BETA
rheology
scanning electron microscopy
MDS
scanning
X-ray
infrared spectroscopy
circular dichroism

Software Mentioned

Origin
AxioVision
STAR e
Nanoindenter
Dichroweb
Agilent GPC / SEC

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