PMID: 8589337Jan 1, 1995Paper

New observations on cytoskeleton and fenestrae in isolated rat liver sinusoidal endothelial cells

Journal of Gastroenterology and Hepatology
F BraetE Wisse

Abstract

Fenestrae control the exchange of fluids, dissolved compounds and small particles between the blood and the space of Disse, and are primarily limited at one side by parenchymal cells. We recently described a simple and rapid method for the isolation, purification and cultivation of rat liver sinusoidal endothelial cells. With regard to the purity and morphology of liver endothelial cells, a detailed microscopic study was performed. Purity and viability after selective adherence was 74 and 95%, respectively. Liver endothelial cell purity was further enhanced to about 95% during adherence and spreading on collagen after 8 h of culture. Liver endothelial cells isolated by this method provide a viable cell population, enabling the study of structure and function of these cells in vitro. We investigated the cytoskeleton associated with fenestrae and sieve plates of liver endothelial cells. Cultured cells were slightly fixed and treated with cytoskeleton extraction buffer containing 0.1% Triton. Whole mounts of extracted liver endothelial cells were prepared for scanning and transmission electron microscopy. Extracted liver endothelial cells show an integral, intricate cytoskeleton. Sieve plates and fenestrae are clearly delineated b...Continue Reading

References

Dec 27, 1977·Biophysics of Structure and Mechanism·H Formanek
May 1, 1976·Experimental Cell Research·D L Knook, E C Sleyster
Jul 1, 1990·Progress in Cardiovascular Diseases·M E Billingham
Nov 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M SteffanA Kirn
Jul 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E WisseR S McCuskey

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Citations

Jan 1, 2013·Computational and Structural Biotechnology Journal·Victoria C CoggerDavid G Le Couteur
Jul 9, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Rajkumar CheluvappaDavid G Le Couteur
Aug 23, 2001·The Journal of Surgical Research·J C GerlachP Neuhaus
Oct 1, 2019·Traffic·Bartlomiej ZapotocznyMarek Szymonski
May 7, 2020·American Journal of Physiology. Cell Physiology·Xinghui Sun, Edward N Harris
May 20, 2020·Biophysics Reviews·Bartlomiej ZapotocznyMarek Szymonski
Nov 7, 2016·Experimental Cell Research·Jeremy MeyerLéo Bühler

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