New olivosyl derivatives of methymycin/pikromycin from an engineered strain of Streptomyces venezuelae

FEMS Microbiology Letters
Jay Sung Joong HongYeo Joon Yoon

Abstract

A mutant strain of Streptomyces venezuelae was engineered by deletion of the entire gene cluster related to biosynthesis of the endogenous deoxysugar (TDP-D-desosamine) and replacement with genes required for biosynthesis of an intermediate sugar (TDP-4-keto-6-deoxy-D-glucose) or an exogenous sugar (TDP-D-olivose), from the oleandomycin and urdamycin deoxysugar pathways. The 'sugar-flexible' glycosyltransferase (DesVII) was able to attach the intermediate sugar and the new sugar to both 12- and 14-membered macrolactones thus producing quinovose or olivose glycosylated 10-deoxymethynolide and narbonolide, respectively. In addition, hydroxylated analogs of the new metabolites were detected. These results demonstrate a successful attempt of engineering the deoxysugar pathway for generation of novel hybrid macrolide antibiotics.

Citations

Aug 3, 2010·Applied Biochemistry and Biotechnology·Nagendra Prasad KurumbangJae Kyung Sohng
Nov 15, 2008·Chemical Communications : Chem Comm·Je Won ParkYeo Joon Yoon
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Je Won ParkYeo Joon Yoon
Feb 26, 2013·Applied and Environmental Microbiology·Aamir GhafoorBernd H A Rehm
Mar 5, 2015·Chemical Society Reviews·Sherif I ElshahawiJon S Thorson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.