New parameters available on Sysmex XE-5000 hematology analyzers contribute to differentiating dengue from leptospirosis and enteric fever

International Journal of Laboratory Hematology
A OehadianB Alisjahbana

Abstract

Distinguishing dengue virus infection from other febrile thrombocytopenic illnesses such as leptospirosis or enteric fever is important but difficult, due to the unavailability of reliable diagnostic tests. Sysmex XE-5000 hematology analyzers use fluorescence flow cytometry to quantitate new parameters including cells in the atypical lymphocyte area (AL), high-fluorescent lymphocyte counts (HFLC), immature granulocytes (IG), and immature platelets (IPF). This study aimed to investigate whether these parameters can help to discriminate between the diseases. We compared hematocytometry performed by a Sysmex XE-5000 analyzer in Indonesian adults with dengue (n = 93), leptospirosis (n = 11), and enteric fever (n = 6) infection, and in healthy controls (n = 28). Receiver operating characteristic curves comparing dengue and leptospirosis showed that dengue was characterized by increased %AL (AUC 0.87; 95% CI 0.70-1.03), %HFLC (AUC 0.89; 95% CI 0.78-0.99), and %IPF (AUC 0.81; 95% CI 0.65-0.97), while patients with leptospirosis had increased %IG (AUC 0.86; 95% CI 0.71-1.02). Low %AL, %HFLC, and %IG supported a diagnosis of enteric fever. The detection of AL, HFLC, IG, and IPF by Sysmex XE-5000 hematology analyzers can help to differen...Continue Reading

References

Jul 1, 1991·The American Journal of Tropical Medicine and Hygiene·E H Yamashiro-KanashiroA J Duarte
Jul 1, 1982·The American Journal of Tropical Medicine and Hygiene·C N EdwardsC O Everard
Oct 1, 1995·Tropical Doctor·S N KhoslaA Khosla
Mar 1, 1994·The Pediatric Infectious Disease Journal·R P WackJ W Bass
Apr 1, 1997·Anesthesia and Analgesia·C Stein
Aug 1, 1997·The Journal of Infectious Diseases·S KalayanaroojF A Ennis
Jan 5, 2002·Microbes and Infection·M M LevineM B Sztein
Sep 12, 2003·Emerging Infectious Diseases·Eva HarrisAngel Balmaseda
Dec 4, 2003·The Lancet Infectious Diseases·Ajay R BhartiUNKNOWN Peru-United States Leptospirosis Consortium
Aug 2, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·David ChadwickNicholas Paton
Aug 30, 2005·Lancet·M K BhanShinjini Bhatnagar
Nov 8, 2005·Lancet·Buddha Basnyat
Dec 28, 2007·PLoS Neglected Tropical Diseases·Daniel H LibratyFrancis A Ennis
Sep 23, 2008·Tropical Medicine & International Health : TM & IH·James A Potts, Alan L Rothman
Nov 26, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K T D ThaiP J de Vries
Aug 24, 2011·Revista Da Sociedade Brasileira De Medicina Tropical·Paulo Vieira DamascoMartha Maria Pereira
Feb 7, 2012·Lancet·Christopher J L MurrayAlan D Lopez
Feb 24, 2012·Transactions of the Royal Society of Tropical Medicine and Hygiene·René te WittPerry J van Genderen
Apr 13, 2012·The New England Journal of Medicine·Cameron P SimmonsBridget Wills
Dec 4, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tatiana M Garcia-BatesSimon M Barratt-Boyes
Feb 19, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F ChappuisC P Yansouni
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Jun 21, 2013·PLoS Neglected Tropical Diseases·Meta MichelsAndré J A M van der Ven
Oct 15, 2013·Thrombosis Research·Ampaiwan Chuansumrit, Wathanee Chaiyaratana
Jan 22, 2014·BMC Infectious Diseases·Andrea L ConroyKevin C Kain
Jun 19, 2014·Critical Reviews in Immunology·Eduardo J M NascimentoSimon M Barratt-Boyes

❮ Previous
Next ❯

Citations

Dec 11, 2019·International Journal of Laboratory Hematology·Liang FuFangyin Zeng
May 3, 2019·PloS One·Choong Shi Hui ClariceEng Soo Yap
Jan 12, 2020·Transactions of the Royal Society of Tropical Medicine and Hygiene·Visula AbeysuriyaSanjay de Mel
Jul 9, 2021·International Journal of Inflammation·Anna Kowalska-KępczyńskaHelena Donica

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.