New PCR-based methods for yeast identification

Journal of Applied Microbiology
Núria HierroJose M Guillamón

Abstract

To characterize reference yeast strains and identify indigenous strains isolated from wine fermentations by PCR methods. We compared several PCR techniques for yeast identification. We used oligonucleotide primers that are complementary to (i) intron splice sites, (ii) REP and (iii) ERIC elements to produce PCR fingerprints that display specific patterns between the different yeast species. These three techniques were used to characterize 41 reference yeast strains belonging to 15 different species and to identify 40 indigenous strains isolated from grape must and wine fermentations. Species-specific banding patterns were obtained with the three PCR-techniques with different degrees of intraspecific differentiation depending on the method. By comparing the PCR fingerprints of unknown isolates with those produced by reference strains, we identified yeast strains isolated from an industrial wine fermentation. All three PCR techniques are rapid, reliable and simple methods of yeast identification. As far as we know, this is the first time that the primers designed for amplifying repetitive elements in bacteria have been successfully used in yeast. Industry needs rapid, reliable and simple methods of yeast identification. The propo...Continue Reading

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Citations

Jun 1, 2010·Food Microbiology·K ZottI Masneuf-Pomarede
Oct 22, 2010·Fungal Biology·Nguyen V TruongLester W Burgess
Nov 20, 2015·The Journal of General and Applied Microbiology·Pratiksha SinghRajesh Kumar Singh
Feb 13, 2007·Research in Microbiology·Artur AlvesAntónio Correia
Oct 17, 2006·Journal of Applied Microbiology·C GuoN Jiang
Nov 4, 2005·Analytical Biochemistry·José Luis Ruiz-BarbaRufino Jiménez-Díaz
Jun 7, 2018·Rapid Communications in Mass Spectrometry : RCM·Francisco Javier De la Torre GonzálezManuel Reinhart Kirchmayr
Mar 15, 2005·Yeast
Nov 23, 2018·Genome Génome / Conseil National De Recherches Canada·Wieland MeyerUNKNOWN ISHAM barcoding of pathogenic fungi working group
May 20, 2017·Frontiers in Microbiology·José M Guillamón, Eladio Barrio
Jul 20, 2021·Biotechnology Reports·Larralde-Corona Claudia PatriciaNarváez-Zapata José Alberto

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