New planar substrates for the in situ synthesis of peptide arrays

Molecular Diversity
Norbert Zander

Abstract

The performance of new cellulose membranes, aminofunctionalized via a PEG spacer, as a solid support in the synthesis of peptide arrays is described. The new membranes are stable to trifluoroacetic acid (TFA) and strong aqueous base for days. These properties extend the scope of synthesis considerably, e.g., more efficient side chain cleavage protocols can be applied which yielded peptides of improved purity. For the first time, cellulose membranes with a loading as high as 5 micromol/cm2 were accessible. Additionally, newly developed polypropylene membranes with hydroxy- or amino functionalities were successfully employed for the SPOT synthesis of peptides and phosphopeptides. The membranes are compatible with antibody binding as well as enzymatic phosphorylation assays.

Citations

Sep 30, 2006·Chemical Communications : Chem Comm·Qi Lin, Helen E Blackwell
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Kai HilperRobert E W Hancock
Sep 28, 2010·Chembiochem : a European Journal of Chemical Biology·Elvira RuppelRudolf Volkmer
May 14, 2009·Chembiochem : a European Journal of Chemical Biology·Rudolf Volkmer
Feb 13, 2018·Journal of Peptide Science : an Official Publication of the European Peptide Society·Justyna FraczykZbigniew J Kaminski
May 26, 2017·Biomedicines·Yosi GiladGary Gellerman

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