New possibilities of accurate particle characterisation by applying direct boundary models to analytical centrifugation

Nanoscale
Johannes WalterWolfgang Peukert

Abstract

Analytical centrifugation (AC) is a powerful technique for the characterisation of nanoparticles in colloidal systems. As a direct and absolute technique it requires no calibration or measurements of standards. Moreover, it offers simple experimental design and handling, high sample throughput as well as moderate investment costs. However, the full potential of AC for nanoparticle size analysis requires the development of powerful data analysis techniques. In this study we show how the application of direct boundary models to AC data opens up new possibilities in particle characterisation. An accurate analysis method, successfully applied to sedimentation data obtained by analytical ultracentrifugation (AUC) in the past, was used for the first time in analysing AC data. Unlike traditional data evaluation routines for AC using a designated number of radial positions or scans, direct boundary models consider the complete sedimentation boundary, which results in significantly better statistics. We demonstrate that meniscus fitting, as well as the correction of radius and time invariant noise significantly improves the signal-to-noise ratio and prevents the occurrence of false positives due to optical artefacts. Moreover, hydrodyna...Continue Reading

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Citations

Feb 22, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Johanna MideletMartinus H V Werts
Jul 3, 2021·International Journal of Molecular Sciences·Abdulkader BakiRegina Bleul
Jul 13, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Kate L ThompsonSteven P Armes
Aug 29, 2019·ACS Omega·Maje Alhaji Haruna, Dongsheng Wen
Dec 18, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Saul J HunterSteven P Armes

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