PMID: 6540134Jul 1, 1984Paper

New possibilities of cytostatic drug testing on patient tumor cells by flow cytometry

Blut
G ValetH Kahle

Abstract

A new assay for cytostatic drug testing is described which can be automated. Pleural effusions and ascites are cultured as such for one week. Cells of solid tumors are cultured in the patients own serum for the same time. The cells are then stained with the esterase and intracellular pH-indicator dye 1,4-diacetoxy-2,3-dicyano-benzene (ADB) to label vital cells. They are simultaneously stained with propidium iodide (PI) as an indicator for dead cells. Monosized fluorescent latex particles are added as concentration, volume and fluorescence standard. Inflammatory cells can be distinguished in the assay from tumor cells because of their small cell volume. The number of dead and surviving cells is counted by the flow cytometer and a therapeutic index is calculated as ratio between the surviving inflammatory to surviving tumor cells. An important feature of the assay is that the DNA-distribution of the dead cells (e.g. aneuploidy) as well as the functional state of the surviving tumor cells and inflammatory cells can be judged from intracellular esterase activity and intracellular pH.

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Citations

Jan 1, 1993·Biology of the Cell·C Jayat, M H Ratinaud
Nov 1, 1986·Photochemistry and Photobiology·R H PottierL A Beiner
May 1, 1995·In Vitro Cellular & Developmental Biology. Animal·M SheridanT P Hennessy
Aug 1, 1985·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·E K RofstadT Brustad
Jan 1, 1986·Annals of the New York Academy of Sciences·V Kachel
Jan 1, 1988·Cytometry. Supplement : the Journal of the Society for Analytical Cytology·F M WaldmanJ Gray
Nov 11, 2006·Journal of Applied Physiology·Wolfgang M KueblerJahar Bhattacharya

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