New Potent Membrane-Targeting Antibacterial Peptides from Viral Capsid Proteins

Frontiers in Microbiology
Susana A DiasAna S Veiga

Abstract

The increasing prevalence of multidrug-resistant bacteria urges the development of new antibacterial agents. With a broad spectrum activity, antimicrobial peptides have been considered potential antibacterial drug leads. Using bioinformatic tools we have previously shown that viral structural proteins are a rich source for new bioactive peptide sequences, namely antimicrobial and cell-penetrating peptides. Here, we test the efficacy and mechanism of action of the most promising peptides among those previously identified against both Gram-positive and Gram-negative bacteria. Two cell-penetrating peptides, vCPP 0769 and vCPP 2319, have high antibacterial activity against Staphylococcus aureus, MRSA, Escherichia coli, and Pseudomonas aeruginosa, being thus multifunctional. The antibacterial mechanism of action of the two most active viral protein-derived peptides, vAMP 059 and vCPP 2319, was studied in detail. Both peptides act on both Gram-positive S. aureus and Gram-negative P. aeruginosa, with bacterial cell death occurring within minutes. Also, these peptides cause bacterial membrane permeabilization and damage of the bacterial envelope of P. aeruginosa cells. Overall, the results show that structural viral proteins are an abu...Continue Reading

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Citations

Mar 13, 2020·The FEBS Journal·Rita I Aroeira, Miguel A R B Castanho
Jul 11, 2018·Frontiers in Chemistry·Patrícia M CarvalhoMarco M Domingues
May 28, 2019·The Journal of Antimicrobial Chemotherapy·Sandra N PintoAna Salomé Veiga
Jan 28, 2021·The Journal of Antimicrobial Chemotherapy·Mário R FelícioSónia Gonçalves
Apr 30, 2019·International Journal of Food Microbiology·Laila N ShwaikiThibaut L C Thery
Jun 3, 2021·Pharmaceuticals·Malak PirtskhalavaGrigol Managadze
Oct 26, 2021·Tissue Barriers·Idris Zubairu SadiqUmar Aliyu Umar
Aug 25, 2020·Biochimica Et Biophysica Acta. Biomembranes·Katharine HammondBart W Hoogenboom

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Methods Mentioned

BETA
Assay
flow cytometry
AFM

Software Mentioned

JPK SPM
APPNano
CellPPD
AMPA

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