New Prodigiosin Derivatives Obtained by Mutasynthesis in Pseudomonas putida

ACS Synthetic Biology
Andreas S KleinJörg Pietruszka

Abstract

The deeply red-colored natural compound prodigiosin is a representative of the prodiginine alkaloid family, which possesses bioactivities as antimicrobial, antitumor, and antimalarial agents. Various bacteria including the opportunistic human pathogen Serratia marcescens and different members of the Streptomycetaceae and Pseudoalteromonadaceae produce prodiginines. In addition, these microbes generally accumulate many structurally related alkaloids making efficient prodiginine synthesis and purification difficult and expensive. Furthermore, it is known that structurally different natural prodiginine variants display differential bioactivities. In the herein described mutasynthesis approach, 13 different derivatives of prodigiosin were obtained utilizing the GRAS (generally recognized as safe) classified strain Pseudomonas putida KT2440. Genetic engineering of the prodigiosin pathway together with incorporation of synthetic intermediates thus resulted in the formation of a so far unprecedented structural diversity of new prodiginine derivatives in P. putida. Furthermore, the formed products allow reliable conclusions regarding the substrate specificity of PigC, the final condensing enzyme in the prodigiosin biosynthesis pathway ...Continue Reading

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Citations

May 3, 2018·Chembiochem : a European Journal of Chemical Biology·Andreas Sebastian KleinJörg Pietruszka
Jul 20, 2018·PloS One·Jennifer Hage-HülsmannAnita Loeschcke
Feb 15, 2019·Applied Microbiology and Biotechnology·Zhongyu YouWenjing Wu
Aug 23, 2019·Chembiochem : a European Journal of Chemical Biology·Maxime CouturierFinian J Leeper
Oct 16, 2019·Chembiochem : a European Journal of Chemical Biology·Katherine J PicottAvena C Ross
Nov 11, 2019·Applied and Environmental Microbiology·Qingshan LongMeifeng Tao
Jun 15, 2019·Microbial Biotechnology·Linde F C KampersVitor A P Martins Dos Santos
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·San-Lang WangAnh Dzung Nguyen
Jun 27, 2020·Chemical Communications : Chem Comm·Stefanie BrandsUlrich Schwaneberg
Nov 13, 2020·Frontiers in Plant Science·Samer S HabashA Sylvia S Schleker
Nov 20, 2020·ChemMedChem·Lea WinandMarkus Nett
Jun 5, 2020·Current Opinion in Biotechnology·Anita Loeschcke, Stephan Thies
Feb 18, 2021·Chemical Communications : Chem Comm·Stefanie BrandsUlrich Schwaneberg
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lena BerningBjörn Stork
Jul 6, 2021·Essays in Biochemistry·Nora Lisa BitzenhoferAnita Loeschcke
Mar 17, 2020·ACS Omega·Edi SetiyonoTatas Hardo Panintingjati Brotosudarmo
Aug 30, 2019·ACS Synthetic Biology·Benedikt WynandsNick Wierckx
Aug 28, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Guangjian RongJose C Merchuk

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