New properties of the soybean trypsin inhibitor: Inhibition of human neutrophil elastase and its effect on acute pulmonary injury

European Journal of Pharmacology
Jannison K C RibeiroM P Sales

Abstract

Seeds from legumes including the Gilcine max are known to be a rich source of protease inhibitors. The soybean Kunitz trypsin inhibitors (SKTIs) have been well characterised and have been found to exhibit many biological activities. However their effects on inflammatory diseases have not been studied to date. In this study, SKTI was purified using anion exchange chromatography using a Resource Q column. The purified protein was able to inhibit human neutrophil elastase (HNE) and bovine trypsin. Purified SKTI inhibited HNE with an IC(50) value of 8mug or 0.3nM. At this concentration SKTI showed neither cytotoxic nor haemolytic effects on human blood cell populations. SKTI showed no deleterious effects on organs, blood cells or the hepatic enzymes ALT and AST in the mouse model of acute systemic toxicity. Human neutrophils incubated with SKTI released less HNE than control neutrophils when stimulated with PAF or fMLP (83.1% and 70% respectively). These results showed that SKTI affected both pathways of elastase release by PAF and fMLP stimuli, suggesting that SKTI is an antagonist of fMLP/PAF receptors. In an in vivo mouse model of LPS acute lung injury, SKTI significantly suppressed the inflammatory effects caused by elastase in...Continue Reading

References

Jul 1, 1989·Trends in Biochemical Sciences·H Neurath
Mar 1, 1982·The Journal of Clinical Investigation·W W McGuireC G Cochrane
Jan 1, 1995·Respiration; International Review of Thoracic Diseases·G HansenV Wahn
Jan 1, 1994·Progress in Medicinal Chemistry·P R BernsteinJ C Williams
Dec 1, 1993·The American Review of Respiratory Disease·C M O'ConnorM X Fitzgerald
Jun 1, 1993·The American Review of Respiratory Disease·J R GossageJ R Snapper
Jul 1, 1993·Infection and Immunity·P S HiemstraR I Lehrer
Mar 4, 2000·The European Respiratory Journal·Y P MoodleyU G Lalloo
Apr 21, 2001·Journal of Agricultural and Food Chemistry·M Friedman, D L Brandon
Jul 27, 2001·The Journal of Surgical Research·D E CarneyG F Nieman
Jan 26, 2002·Journal of Natural Products·Senia JohanssonLars Bohlin
Mar 14, 2002·Journal of Protein Chemistry·G C MelloM L Macedo
Jun 27, 2002·Expert Opinion on Investigational Drugs·Hiroyuki Ohbayashi
Oct 22, 2003·Molecular Immunology·Deirdre A Devine
Nov 25, 2003·The American Journal of Pathology·Steven D ShapiroAbderazzaq Belaaouaj
Apr 29, 2004·Journal of Agricultural and Food Chemistry·Maria Lígia Rodrigues MacedoJosé Roberto Postali Parra
Jun 9, 2004·Joint, Bone, Spine : Revue Du Rhumatisme·Birkan Sonel TurNurşen Düzgün
Aug 18, 2004·Archives of Biochemistry and Biophysics·Zhong LaiWilliam C Groutas
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·L P A McGarveyJ S Elborn
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Ristan M GreerScott C Bell
Nov 13, 2004·Clinical and Diagnostic Laboratory Immunology·Hidenori MatsuzakiToshihiko Terao
Jun 7, 2007·Digestive Diseases and Sciences·Gary R LichtensteinJeffrey H Ware

❮ Previous
Next ❯

Citations

Nov 4, 2011·Science Translational Medicine·Sarah NavinaVijay P Singh
Jun 9, 2012·Plant Physiology and Biochemistry : PPB·Domenico De PaolaGabriella Sonnante
Mar 12, 2015·Proteomics·Mehran Dastmalchi, Sangeeta Dhaubhadel
Feb 9, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Vanessa Cristina Oliveira de LimaAna Heloneida de Araújo Morais
Apr 16, 2018·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Lynsey D JonesVermont P Dia
Jan 1, 2014·Journal of Agricultural and Food Chemistry·Luciana S GomesEliane Fialho

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here