PMID: 8590475Sep 1, 1995Paper

New protease mutants in Aspergillus niger result in strongly reduced in vitro degradation of target proteins; genetical and biochemical characterization of seven complementation groups

Current Genetics
Johannes P T W van den HomberghJaap Visser

Abstract

Several mutants of Aspergillus niger, deficient in extracellular protease expression, have been isolated and characterized both genetically and biochemically. The mutant strains, obtained after in vivo UV-mutagenesis of conidiospores and selected by haloscreening on a new dual-substrate plate assay, belong to at least seven different complementation groups. These seven prt loci were assigned to linkage groups using master strains with marked chromosomes. One prt locus (prtC) could be assigned to linkage group I, three (prtB, prtE and prtG) to linkage group III, one (prtF) to linkage group V and the two remaining loci (prtA and prtD) to linkage group VIII. Extracellular proteolytic activities varied from 2 to 3% up to 80% of the protease activity of the parental strain. Assigning the different prt mutants to structural or regulatory genes is difficult since only one structural gene, pepA, has been mapped unambiguously on linkage group I but is not identical to prtC. All prt mutants except for prtC are likely to be regulatory mutants or else belong to a proteolytic cascade because residual activities showed that more proteolytic activities were affected simultaneously. Double mutants were constructed both by recombination and by ...Continue Reading

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Citations

Dec 13, 2007·Applied Microbiology and Biotechnology·Qiang LiBrian McNeil
Jul 1, 1997·Trends in Biotechnology·J P van den HomberghJ Visser
Jul 9, 2004·Applied and Environmental Microbiology·Ronald P de VriesJaap Visser
Apr 1, 2014·Ultrasonics·Achim M LoskeMiguel A Gómez-Lim
Jan 1, 1997·Critical Reviews in Biotechnology·D B Archer, J F Peberdy
Oct 2, 2008·Biotechnology and Bioengineering·Liat ChillJoseph Shiloach
Jan 10, 2009·Journal of Molecular Biology·Buvaneswari NarayananOsnat Herzberg
Aug 16, 2001·Biotechnology and Bioengineering·M G WiebeP J Punt
Jun 28, 2014·Biomolecules·Jose L Adrio, Arnold L Demain
Dec 7, 2007·Molecular Biotechnology·Arnold L Demain, Jose L Adrio
Dec 17, 2002·The Journal of General and Applied Microbiology·Norihiro TsukagoshiMasashi Kato
Jan 25, 2007·The Journal of Biological Chemistry·Ying HanDebra Dunaway-Mariano
Jan 22, 1998·FEMS Microbiology Letters·C B FauldsG Williamson

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