PMID: 9421927Jan 9, 1998Paper

New ribosome-inactivating proteins with polynucleotide:adenosine glycosidase and antiviral activities from Basella rubra L. and bougainvillea spectabilis Willd

Planta
Andrea BolognesiFiorenzo Stirpe

Abstract

New single-chain (type 1) ribosome-inactivating proteins (RIPs) were isolated from the seeds of Basella rubra L. (two proteins) and from the leaves of Bougainvillea spectabilis Willd. (one protein). These RIPs inhibit protein synthesis both in a cell-free system, with an IC50 (concentration causing 50% inhibition) in the 10(-10) M range, and by various cell lines, with IC50S in the 10(-8)-10(-6) M range. All three RIPs released adenine not only from rat liver ribosomes but also from Escherichia coli rRNA, polyadenylic acid, herring sperm DNA, and artichoke mottled crinkle virus (AMCV) genomic RNA, thus being polynucleotide:adenosine glycosidases. The proteins from Basella rubra had toxicity to mice similar to that of most type 1 RIPs (Barbieri et al., 1993, Biochim Biophys Acta 1154: 237-282) with an LD50 (concentration that is 50% lethal) < or = 8 mg.kg-1 body weight, whilst the RIP from Bougainvillea spectabilis had an LD50 > 32 mg.kg-1. The N-terminal sequence of the two RIPs from Basella rubra had 80-93% identity, whereas it differed from the sequence of the RIP from Bougainvillea spectabilis. When tested with antibodies against various RIPs, the RIPs from Basella gave some cross-reactivity with sera against dianthin 32, an...Continue Reading

Citations

Mar 16, 2002·European Journal of Biochemistry·Marcel T den HartogFiorenzo Stirpe
Nov 16, 2005·Natural Product Research·András SimonHanan Samir
Aug 4, 2012·Cancer Biotherapy & Radiopharmaceuticals·Joycelyn EntwistleGlen C MacDonald
Apr 7, 2004·European Journal of Biochemistry·Frank VandenbusscheEls J M Van Damme
Nov 10, 2011·Toxins·Maddalena de VirgilioMaria Serena Fabbrini
May 27, 2015·Toxins·Joachim SchrotMatthias F Melzig
Jul 21, 2009·Journal of Structural Biology·Simona FermaniAndrea Bolognesi
Oct 15, 2014·Asian Pacific Journal of Tropical Medicine·Suresh KilledarSachin Pishawikar
Dec 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shilpa ChooniedassJeannick Cizeau
Jan 31, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea BolognesiLetizia Polito
Nov 21, 2019·Current Protein & Peptide Science·Snober Shabeer WaniTanveer A Dar
Feb 5, 2002·The Journal of Biological Chemistry·Andrea BolognesiFiorenzo Stirpe
Aug 12, 2018·Toxins·Massimo BortolottiLetizia Polito
Jan 28, 2021·Toxins·Lucía CitoresJosé M Ferreras

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