New role of human ribosomal protein S3: Regulation of cell cycle via phosphorylation by cyclin-dependent kinase 2

Oncology Letters
Se Hee HanYe Sun Han

Abstract

Human ribosomal protein S3 (hRpS3) is a component of the 40S ribosomal subunit that associated in protein synthesis. hRpS3 has additional ribosomal functions such as DNA repair, transcription, metastasis, and apoptosis via interaction with numerous signaling molecules and has different modifications. Cyclin-dependent kinases (CDKs) are heterodimeric serine/threonine protein kinases that regulate cell cycle progression. Among its members, the Cdk1-cyclin B complex is known to control cell progression in the G2/M phase, while Cdk2-cyclin E/A complexes function in G1/S and S/G2 transition. In our previous study, we observed interaction between hRpS3 and Cdk1. The present study investigated the interaction between hRpS3 and Cdk2. Cdk2 phosphorylated hRps3 at amino acid residues S6 and T221 during the S-phase. Furthermore, hRpS3 knockdown delayed cell cycle progression by modulating the expression of cell cycle-related proteins, including cyclin B1 and cyclin E1. These findings suggest that hRpS3 is involved in Cdk2-mediated cell cycle regulation.

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Citations

Nov 26, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Jing-Wei LiangFan-Hao Meng
Apr 17, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yong Jun ParkJoon Kim
Jan 20, 2021·Cellular and Molecular Life Sciences : CMLS·Y J ParkJ Kim
Mar 8, 2021·Trends in Cell Biology·Marina BuryFrédéric Lessard

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Methods Mentioned

BETA
nuclear translocation
PCR
immunoprecipitation
pull-down
transfection
fluorescence-activated cell sorting
co-IP
FACS
immunoprecipitation assay

Software Mentioned

NetPhos2
Cell Quest Alias
Excel

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