New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue

Frontiers in Immunology
Karl D Brune, Mark Howarth

Abstract

Vaccines based on virus-like particles (VLPs) can induce potent B cell responses. Some non-chimeric VLP-based vaccines are highly successful licensed products (e.g., hepatitis B surface antigen VLPs as a hepatitis B virus vaccine). Chimeric VLPs are designed to take advantage of the VLP framework by decorating the VLP with a different antigen. Despite decades of effort, there have been few licensed chimeric VLP vaccines. Classic approaches to create chimeric VLPs are either genetic fusion or chemical conjugation, using cross-linkers from lysine on the VLP to cysteine on the antigen. We describe the principles that make these classic approaches challenging, in particular for complex, full-length antigens bearing multiple post-translational modifications. We then review recent advances in conjugation approaches for protein-based non-enveloped VLPs or nanoparticles, to overcome such challenges. This includes the use of strong non-covalent assembly methods (stick), unnatural amino acids for bio-orthogonal chemistry (click), and spontaneous isopeptide bond formation by SpyTag/SpyCatcher (glue). Existing applications of these methods are outlined and we critically consider the key practical issues, with particular insight on Tag/Catc...Continue Reading

References

Dec 13, 2002·Nature·Theodore Jardetzky
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Mar 23, 2004·Nature Biotechnology·Emmanuel G GuignetHorst Vogel
Mar 18, 2005·Journal of the American Chemical Society·Tara L SchlickMatthew B Francis
Apr 21, 2005·The Journal of Biological Chemistry·Sau-Ching Wu, Sui-Lam Wong
May 7, 2005·Nature Biotechnology·Thomas A KostDonald L Jarvis
Aug 24, 2007·Traffic·Dieuwke EngelsmaMaarten Fornerod
Oct 28, 2008·Biological Chemistry·Gary T Jennings, Martin F Bachmann
Feb 10, 2009·Current Topics in Microbiology and Immunology·E Strable, M G Finn
Apr 30, 2009·Current Topics in Microbiology and Immunology·M L SmithK E Palmer
May 7, 2009·Annual Review of Biophysics·Thomas C TerwilligerShigeyuki Yokoyama
Oct 24, 2009·Biochemistry·Steven D BrownM G Finn
Mar 20, 2010·Journal of the American Chemical Society·Bijan Zakeri, Mark Howarth
Jul 24, 2010·Cancer Immunology, Immunotherapy : CII·Sharmila Pejawar-GaddyIoannis Bossis
Aug 28, 2010·Journal of Biotechnology·Mervyn W O LiewAnton P J Middelberg
Oct 16, 2010·Nature Reviews. Immunology·Martin F Bachmann, Gary T Jennings
Nov 17, 2011·Nature Chemical Biology·Nicholas Stephanopoulos, Matthew B Francis
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bijan ZakeriMark Howarth
Apr 20, 2012·Chembiochem : a European Journal of Chemical Biology·Pijus K SasmalEric Meggers
Jul 19, 2012·Biotechnology and Bioengineering·Kok Hong LimSheldon Park
Nov 10, 2012·The New England Journal of Medicine·UNKNOWN RTS,S Clinical Trials PartnershipPreeti Vansadia
Nov 17, 2012·Nature Reviews. Microbiology·Philip R DormitzerRino Rappuoli
Jan 19, 2013·The Journal of Biological Chemistry·Harald Nothaft, Christine M Szymanski
May 1, 2013·Biological Chemistry·Hauke LilieFranziska Gehle
Sep 18, 2013·Molecular & Cellular Proteomics : MCP·Cécile CrosnierGavin J Wright
Sep 24, 2013·Journal of Molecular Biology·Michael FairheadMark Howarth
Nov 28, 2013·Angewandte Chemie·Ron M VersteegenMarc S Robillard
Jan 18, 2014·Analytical Chemistry·Bethany C GrossDana M Spence
Mar 19, 2014·Chemical Science·Neel H Shah, Tom W Muir
Jun 14, 2014·Nature Reviews. Immunology·Ennio De Gregorio, Rino Rappuoli

❮ Previous
Next ❯

Citations

Mar 16, 2019·Scientific Reports·Anne-Marie C AnderssonMark Howarth
Jan 1, 2020·Expert Review of Vaccines·Lasse NeukirchPeter Johannes Holst
Apr 17, 2019·Nature Communications·Irsyad N A Khairil AnuarMark Howarth
Jul 17, 2020·Nature Nanotechnology·Matthew D ShinNicole F Steinmetz
Mar 11, 2020·Frontiers in Immunology·Kyle SaylorChenming Zhang
May 6, 2019·International Journal of Molecular Sciences·Daniel HatlemJack C Leo
May 30, 2020·Nanomaterials·Ximena ZottigSteve Bourgault
Nov 8, 2018·Toxins·Erick Bermúdez-MéndezAndreas Hougaard Laustsen
Dec 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Anthony H KeebleMark Howarth
Sep 5, 2020·Angewandte Chemie·Rolle RahikainenMark Howarth
Feb 6, 2021·Virology·Christian Isalomboto Nkanga, Nicole F Steinmetz
Nov 17, 2020·Allergy, Asthma & Immunology Research·Phornsiri PechsrichuangAlain Jacquet
Feb 9, 2021·Chemical Science·Anthony H Keeble, Mark Howarth
Nov 3, 2020·Frontiers in Immunology·Ayat Zawawi, Kathryn J Else
Apr 15, 2020·Protein Expression and Purification·Ario de Marco
May 15, 2021·NPJ Vaccines·Brian Nguyen, Niraj H Tolia
Jun 1, 2021·ACS Central Science·Yingzhu LiQiongqiong Zhou
Jul 30, 2021·Cell Chemical Biology·Anthony H KeebleMark Howarth
Apr 3, 2020·ACS Applied Materials & Interfaces·Jhanvi SharmaTrevor Douglas

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
dissection
acylation
peptide display

Software Mentioned

Catcher
SnoopCatcher
SpyTag
SnoopTag
Tag
SpyCatcher

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.