New screening test to determine the acceptability of 0.45-micron membrane filters for analysis of water.

Applied and Environmental Microbiology
K P Brenner, C C Rankin

Abstract

During routine membrane filter (MF) quality control testing, irregularities such as partial or complete inhibition of microbial growth at grid lines, abnormal spreading of colonies, growth in or along the grid lines, nonwetting areas, poor colony sheen and metallic sheen on the MF surface with mEndo agar, brittleness, decreased recovery, and severe wrinkling were observed with several lots of filters. To study these effects and to develop a more sensitive screening test for MF quality, we compared five different MFs with various types and degrees of defects by using five stock coliform cultures and five different media. Results showed that the Enterobacter aerogenes-tryptic soy agar test system detected more MF defects than any other combination did and was superior to the Escherichia coli-mFC agar American Society for Testing and Materials method for grid line inhibition. Filtered natural samples grown on the same media showed the same effects as were observed with the pure cultures. Poor colony sheen and sheen on the MF surface were best detected with Enterobacter aerogenes on mEndo agar. The use of tryptic soy agar and mEndo agar with this organism permitted the maximum detection of MF irregularities. Of the 142 MF lots test...Continue Reading

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Citations

Nov 28, 2013·Critical Reviews in Microbiology·Martin J AllenSteve E Hrudey
Mar 3, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H SimşekH Vahaboğlu
Jun 3, 2006·Environmental Monitoring and Assessment·Robin W KlootDaniel Duke Brantley
Jan 1, 1993·Applied and Environmental Microbiology·L SmithS Mottice
Apr 7, 2010·Applied and Environmental Microbiology·Shahar RotemAmram Mor

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