New series of lipoxins isolated from human eosinophils

FEBS Letters
D Steinhilber, H J Roth

Abstract

Granulocytes from human eosinophilic donors were incubated with arachidonic acid or 15-hydroxyeicosatetraenoic acid (15-HETE) and stimulated with the ionophore A23187. The eicosanoids were extracted with reversed-phase cartridges and subjected to RP-HPLC analysis. When extracts from eosinophil-enriched populations were analysed and compared with extracts from human neutrophils, three additional peaks were detected which coeluted with 15-hydroxy-delta 13-trans-15H derivatives of leukotriene C4, D4 and E4 in different HPLC systems. The recorded absorbance spectra of the eluted compounds and the standards were identical and showed a maximum at 307 nm which is characteristic for a conjugated tetraene system with a bathochromic shift by the sulfur moiety in alpha-position to the tetraene system. The compound which coeluted with the 15-hydroxy-LTC4 standard was treated with gamma-glutamyltransferase and converted to the corresponding leukotriene D4 derivative. The results indicate that interaction between the 5- and 15-lipoxygenase pathways leads to the formation of a new series of arachidonic acid metabolites in human eosinophils. Since the biosynthetic route is similar to that of lipoxin A4 and lipoxin B4, we suggest the trivial na...Continue Reading

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Citations

Jul 30, 1991·Biochimica Et Biophysica Acta·A F Rowley
Apr 14, 1994·Biochimica Et Biophysica Acta·C N Serhan
Apr 18, 1991·The New England Journal of Medicine·P F Weller
Aug 9, 2007·International Archives of Allergy and Immunology·Takehito KobayashiMakoto Nagata
Sep 26, 2000·Pulmonary Pharmacology & Therapeutics·M A GiembyczM A Lindsay
Oct 15, 1991·Biochemical and Biophysical Research Communications·L StenkeJ A Lindgren
May 1, 1993·Journal of Comparative Pathology·D G Jones

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