New slbo-Gal4 driver lines for the analysis of border cell migration during Drosophila oogenesis

Chromosoma
Anna A OgienkoElina M Baricheva

Abstract

Border cell (BC) migration during Drosophila oogenesis is an excellent model for the analysis of the migratory and invasive cell behavior. Most studies on BC migration have exploited a slbo-Gal4 driver to regulate gene expression in these cells or to mark them. Here, we report that the slbo-Gal4 transgene present in the line #6458 from the Bloomington Stock Center is inserted within chickadee (chic), a gene encoding the actin-binding protein Profilin, which promotes actin polymerization and is known to be involved in cell migration. The chic6458 mutation caused by the transgene insertion behaves as a null chic allele and is homozygous lethal. To evaluate possible effects of chic6458 on the assessment of BC behavior, we generated new lines bearing the slbo-Gal4 transgene inserted into different second chromosome loci that do not appear to be involved in cell migration. Using these new lines and the slbo-Gal4-chic6458 line, we defined the functional relationships between the twinfilin (twf) and chic in BC migration. Migration of BCs is substantially reduced by mutations in twf, which encodes an actin-binding protein that inhibits actin filament assembly. The defects caused by twf mutations are significantly suppressed when the sl...Continue Reading

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Citations

Oct 24, 2020·BMC Genetics·Tatiana V TatarinovaYuriy L Orlov

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Datasets Mentioned

BETA
KY800902-KY800905

Methods Mentioned

BETA
transgenic
Scanning electron microscopy
PCR
electrophoresis
genotyping
scanning electron

Software Mentioned

CFX [UNK]
slbo
Gal4
LSM Image Browser

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