New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity

Journal of the National Cancer Institute
O S WeislowM R Boyd

Abstract

We have developed an effective and optimally safe microculture method for rapid and convenient assay of the in vitro cytopathic effects of human immunodeficiency virus (HIV-1) on human lymphoblastoid or other suitable host cells. The assay procedure is applicable to the evaluation of drug effects on in vitro infections induced directly in cultured host cells by cell-free HIV-1 or by coculture with H9 cells chronically infected with HIV-1. The assay uses a newly developed tetrazolium reagent that is metabolically reduced by viable cells to yield a soluble, colored formazan product measurable by conventional colorimetric techniques. This simple microassay minimizes the number of plate manipulations typically required with other assay methods and, coupled with computerized data collection and analysis, facilitates large-scale screening of agents for potential antiviral activity. To support and enhance the discovery of new anti-HIV-1 agents, the National Cancer Institute is offering investigators worldwide the opportunity to submit new candidate agents for anti-HIV-1 screening with this method.

Citations

Jun 1, 1995·Journal of Neuroscience Research·F C Martin, C A Wiley
Jan 7, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Mati FridkinRaniero Rocchi
Jan 1, 1992·Cancer Chemotherapy and Pharmacology·S B Sobottka, M R Berger
Nov 15, 2002·Journal of Biomedical Science·Chun-Ching LinTa-Chen Lin
Jul 1, 2011·Chinese Journal of Integrative Medicine·Ji PangJun-Wen Li
Jun 1, 1991·Journal of Virological Methods·R J GulakowskiM R Boyd
Oct 1, 1993·Antiviral Research·S J SperberS Pestka
May 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·O I AruomaB Halliwell
Oct 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H BabichA Stern
Feb 1, 1995·Bioorganic & Medicinal Chemistry·H DuludeG Gallant
Feb 26, 2004·Bioorganic & Medicinal Chemistry Letters·Dharmarajan SriramTanushree Ratan Bal
May 4, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S L Holbeck
Feb 28, 2004·European Journal of Medicinal Chemistry·Luisa SaviniM Barbara Pisano
Nov 11, 2003·Journal of Microbiological Methods·Cornelly WilliamsLuz I Romero
Sep 5, 2002·Biochemical and Biophysical Research Communications·Andrew G StephenRobert J Fisher
Aug 29, 2001·Phytochemistry·K P ManfrediL K Pannell
Mar 19, 2003·Bioorganic Chemistry·A Geoffrey SkillmanGeorge L Kenyon
Mar 15, 2001·Antiviral Research·L SharmeenS Gracheck
Jun 22, 2002·Antiviral Research·L C ChiangC C Lin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.