New stereoscopic reconstruction protocol for scanning electron microscope images and its application to in vivo replicas of the shoot apical meristem

Functional Plant Biology : FPB
Anne-Lise Routier-Kierzkowska, Dorota Kwiatkowska

Abstract

The shoot apical meristem is a small and delicate organ, usually hidden between the young leaves and flowers that it produces. One approach to study meristem geometry and growth consists of taking consecutive replicas from the living meristem surface. In this paper, we present a new stereoscopic reconstruction method for this non-invasive replica protocol, which is applicable to study of growth and geometry of individual cells. This method had been used by the authors to study shoot apical meristem of two species: Arabidopsis thaliana (L.) Heynh. and Anagallis arvensis L., and can be extended to other species and organs. Scanning electron micrographs of the same replica are made at two different angles of view. The obtained stereopairs are used for the dense, three dimensional reconstruction of the replica surface. At the same time, some of the microscope parameters are refined based on the differences between the two micrographs. Three dimensional cell outlines are next extracted from the dense continuous reconstruction, and provide a basis for the quantification of meristem geometry and growth. The new reconstruction protocol can be used with different types of scanning electron microscopes, single- or multi-staged, does not ...Continue Reading

References

Jul 18, 2002·The Plant Journal : for Cell and Molecular Biology·Jacques Dumais, Dorota Kwiatkowska
May 6, 2003·Journal of Experimental Botany·Dorota Kwiatkowska, Jacques Dumais
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology·Pierre Barbier de ReuilleJan Traas
Dec 28, 1962·Science·P B Green
May 1, 1987·IEEE Transactions on Pattern Analysis and Machine Intelligence·K S ArunS D Blostein

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Citations

Jun 27, 2018·Journal of Experimental Botany·Marcin LipowczanDorota Kwiatkowska
May 7, 2015·ELife·Pierre Barbier de ReuilleRichard S Smith
Dec 17, 2009·The New Phytologist·Olivier Hamant, Jan Traas
Jun 11, 2009·Journal of Experimental Botany·Dorota Kwiatkowska, Anne-Lise Routier-Kierzkowska
Jun 2, 2009·The Plant Cell·Md Rezaul KarimMitsuhiro Aida

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