PMID: 9183433Apr 1, 1997Paper

New strategy for RNA vectorization in mammalian cells. Use of a peptide vector

Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
P VidalGilles Divita

Abstract

A major barrier for gene delivery is the low permeability of nucleic acids to cellular membranes. The development of antisenses and gene therapy has focused mainly on improving methods of oligonucleotide or gene delivery to the cell. In this report we described a new strategy for RNA cell delivery, based on a short single peptide. This peptide vector is derived from both the fusion domain of the gp41 protein of HIV and the nuclear localization sequence of the SV40 large T antigen. This peptide vector localizes rapidly to the cytoplasm then to the nucleus of human fibroblasts (HS-68) within a few minutes and exhibits a high affinity for a single-stranded mRNA encoding the p66 subunit of the HIV-1 reverse transcriptase (in a 100 nM range). The peptide/RNA complex formation involves mainly electrostatic interactions between the basic residues of the peptide and the charges on the phosphate group of the RNA. In the presence of the peptide-vector fluorescently-labelled mRNA is delivered into the cytoplasm of mammalian cells (HS68 human fibroblasts) in less than 1 h with a relatively high efficiency (80%). This new concept based on a peptide-derived vector offers several advantages compared to other compounds commonly used in gene de...Continue Reading

References

Jan 11, 1977·Biochemistry·J C MatthewsM J Cormier
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Dec 1, 1995·The Journal of Biological Chemistry·G DivitaR S Goody
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Oct 1, 1996·Trends in Genetics : TIG·J D Harris, N R Lemoine
Oct 1, 1996·Trends in Biotechnology·S Agrawal

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Citations

Feb 14, 1998·FEBS Letters·J DufourcqN Henry-Toulmé

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