New structure and function in plant K+ channels: KCO1, an outward rectifier with a steep Ca2+ dependency

The EMBO Journal
Katrin CzempinskiB Müller-Röber

Abstract

Potassium (K+) channels mediating important physiological functions are characterized by a common pore-forming (P) domain. We report the cloning and functional analysis of the first higher plant outward rectifying K+ channel (KCO1) from Arabidopsis thaliana. KCO1 belongs to a new class of 'two-pore' K+ channels recently described in human and yeast. KCO1 has four putative transmembrane segments and tandem calcium-binding EF-hand motifs. Heterologous expression of KCO1 in baculovirus-infected insect (Spodoptera frugiperda) cells resulted in outwardly rectifying, K+-selective currents elicited by depolarizing voltage pulses in whole-cell measurements. Activation of KCO1 was strongly dependent on the presence of nanomolar concentrations of cytosolic free Ca2+ [Ca2+]cyt. No K+ currents were detected when [Ca2+]cyt was adjusted to <150 nM. However, KCO1 strongly activated at increasing [Ca2+]cyt, with a saturating activity observed at approximately 300 nM [Ca2+]cyt. KCO1 single channel analysis on excised membrane patches, resulting in a single channel conductance of 64 pS, confirmed outward rectification as well as Ca2+-dependent activation. These data suggest a direct link between calcium-mediated signaling processes and K+ ion tr...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J A AndersonR F Gaber
Feb 1, 1991·The Journal of Membrane Biology·K A Ketchum, R J Poole
Aug 1, 1991·Current Opinion in Cell Biology·T M Perney, L K Kaczmarek
Jan 1, 1987·The Journal of Membrane Biology·T Iijima, S Hagiwara
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·J I SchroederW Gassmann
Feb 23, 1996·The Journal of Biological Chemistry·F LesageJ Barhanin
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·S A GoldsteinM H Pausch

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Citations

Mar 29, 2008·European Biophysics Journal : EBJ·Alexandra DedmanEric Honoré
Sep 1, 2005·Pflügers Archiv : European journal of physiology·A RollerA Bertl
Jun 1, 2005·Current Opinion in Neurobiology·Leigh D PlantSteve A N Goldstein
Dec 5, 2003·Trends in Pharmacological Sciences·Donghee Kim
Apr 16, 2002·Trends in Plant Science·Anne Aliénor Véry, Hervé Sentenac
Mar 6, 1999·Current Opinion in Plant Biology·S RounsleyK A Ketchum
Mar 6, 1999·Current Opinion in Plant Biology·E Grill, A Himmelbach
Dec 23, 1999·Current Opinion in Plant Biology·S Zimmermann, H Sentenac
Sep 7, 2001·Plant, Cell & Environment·E P Spalding
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·Q Bei, S Luan
Aug 1, 2002·The Plant Journal : for Cell and Molecular Biology·Katrin CzempinskiBernd Mueller-Roeber
Oct 31, 2000·The Journal of General Physiology·N ZilberbergS A Goldstein
Feb 14, 2002·Plant Physiology·Menachem MoshelionNava Moran
Aug 23, 2005·Plant Physiology·Hermann BihlerAdam Bertl
Jul 4, 2006·Plant Physiology·Mark W SzczerbaHerbert J Kronzucker
Aug 14, 2001·Plant Physiology·P MäserM L Guerinot
Jul 22, 1999·Annals of the New York Academy of Sciences·Z W WangL Salkoff
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Tama Christine Fox, Mary Lou Guerinot
Sep 12, 2002·Annual Review of Plant Biology·Vadim DemidchikMark Tester
Sep 25, 2003·Annual Review of Plant Biology·Anne-Aliénor Véry, Hervé Sentenac
Aug 13, 2009·International Journal of Plant Genomics·Filiz Yesilirmak, Zehra Sayers
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z M PeiJ I Schroeder
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·D BeckerR Hedrich
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Julian I Schroeder
Apr 10, 2007·FEBS Letters·Anne LebaudyHervé Sentenac
Mar 5, 2016·Frontiers in Plant Science·Nasser SewelamPeer M Schenk
Mar 8, 2011·Cell Calcium·Edgar Peiter
Jun 9, 2009·Current Opinion in Plant Biology·Sheng LuanSung Chul Lee
Feb 17, 2009·Journal of Plant Physiology·Mark W SzczerbaHerbert J Kronzucker
Mar 18, 2008·Progress in Biophysics and Molecular Biology·Joost H A FolgeringEric Honoré

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